Showing papers in "Journal of Microbiological Methods in 2003"

TL;DR: This work re-assess the specificity of commonly used 16S rRNA gene primers and presents these data in tabular form designed as a tool to aid simple analysis, selection and implementation.

TL;DR: CaO was the most effective, followed by MgO and ZnO, against E. coli, and was suggested to have a strong affinity to the cells of S. aureus.

TL;DR: Bacterial cytoplasmic membrane changes and related functional effects will be examined as well as the use of fluorescence polarization methods and examples of data obtained from research with bacteria.

TL;DR: This review assesses the molecular methods currently available and evaluates their ability to assess cell viability with emphasis on environmental pathogens and offers speed, sensitivity and specificity.

TL;DR: A protocol was developed using 96-well plates and multichannel pipettes for serial dilutions, followed by drop plating on agar in a 6 x 6 format which greatly decreases the number of plates utilized thereby saving incubator space for organisms such as Campylobacter.

TL;DR: A quantitative spectrophotometric method was developed to measure the removal and killing efficacy of antibiofilm agents and is sensitive enough to elucidate concentration-response relationships as well as differences between species responses to treatments.

TL;DR: A critical review of different strategies which have been employed for the purification of bacterial, yeast and fungal lipases and novel purification technologies now available in this field are reviewed.

TL;DR: It is demonstrated that the Luminex LabMAP system is a rapid, flexible platform capable of simultaneous, sensitive and specific detection of pathogens.

TL;DR: The present review summarizes the state of the art in development of the probes for detection of the biological threat agents: toxins, bacteria, spores and viruses.

TL;DR: A new method for rapid gene disruption in Y. lipolytica is described, which combines SEP method and the Cre-lox recombination system, facilitating efficient marker rescue and is an ideal tool for the functional analysis of gene families, or for creating large-scale mutant collections in general.

TL;DR: Microarrays can be coupled with PCR where they serve as a set of parallel dot-blots to enhance product detection and identification and can be used to identify diagnostic markers suitable for developing new PCR-based detection assays.

TL;DR: The rapidity and feasibility of the TaqMan method point to benefits in drinking water analysis, particularly in emergency situations and, thus, to improved public health management.

TL;DR: It is proposed that it is essential to screen 16S rRNA gene products for bacterial diversity by DGGE or other rapid fingerprinting methods, prior to their use in establishing a representative clone library of deep sub-seafloor bacteria.

TL;DR: The initial studies have shown that the Gram-positive Listeria and Gram-negative Legionella bacteria, Bacillus spores and Cryptosporidium oocysts can often be identified at the subspecies/strain level on the basis of SERS fingerprints collected from single organisms.

TL;DR: In this article, chemical flocculation using multivalent cations was investigated as a potential method for eliminating soil-based inhibitors during the extraction process, which significantly reduced the co-purification of PCR inhibitors with minimal loss of DNA yield.

TL;DR: Recent advances in sample collection, on-line sample processing and purification, and DNA microarray technologies may form the basis of a universal method to detect known and emerging waterborne pathogens.

TL;DR: Raw and potable water analysis revealed that DVC and culture-based techniques reported significantly fewer viable bacteria compared to the number of physiologically active bacteria detected using the rapid FCM assays, where this difference appeared to be nonlinear across different samples.

TL;DR: It was concluded that viable microbial contamination can be reliably monitored by measurement of intracellular ATP, and that this method may be considered superior to cultivable colony counts due to its speed and its ability to report the presence of viable but noncultivable organisms.

TL;DR: This study demonstrated that real time PCR is a rapid and reliable technique for detecting V. parahaemolyticus possessing the tdh gene in pure cultures and in oyster enrichments.

TL;DR: The modality of acid addition is shown to be the critical step for obtaining reproducible test results between different technicians, and raising the incubation temperature above 80 degrees C increased the consistency of the method by more than 60% regardless of the acid addition modality.

TL;DR: TRF drift was determined for 21 bacterial species using an ABI 310 Genetic Analyzer and it was found that subtle differences in molecular weight, whether from purine content or dye label, can significantly affect the observed TRF length.

TL;DR: Production of extracellular amylase was demonstrated under stress conditions of high temperature and high salinity in aerobically cultivated culture of a newly isolated moderately halophilic bacterium of spore-forming Halobacillus sp.

TL;DR: The DNA extraction protocol and real-time PCR quantification assay can be performed in less than 2 h and is adaptable to detect and quantify genomic DNA from other soilborne fungi.

TL;DR: Although a relatively recent method, NASBA has the potential for adoption as a diagnostic tool for environmental pathogens.

TL;DR: The data suggest the appropriateness of the QIAamp DNA Stool Mini Kit for the studies of gut microbial ecology and the effectiveness of theQIAamp kit in processing multiple samples for cell lysis and DNA extraction and the high quality of DNA extracts generated using the two methods.

TL;DR: The application of the LH-PCR method as a monitoring tool for bioremediation could greatly enhance and extend the current understanding of the microbial community dynamics during the biodegradation of environmental contaminants.

TL;DR: The use of microgravity technology to generate physiologically relevant human tissue models for research in bacterial pathogenesis and the response of bacteria to environmental signals, which are similar to those encountered during prokaryotic life cycles, are discussed.

TL;DR: The data, obtained after application of different methods of investigation and validated with membrane filtration, showed a strong inhibitory effect of basil on the test bacteria, which were considered encouraging.

TL;DR: There was a good correlation between the number of CTC-positive cells and the CFU count, regardless of the growth phase, and CTC could still be reduced by a large part of the population during the first hours of stationary phase even if the bacteria were no longer releasing CO(2).

TL;DR: The results prove that 16S rDNA genotyping in combination with DGGE analysis is a promising approach for the detection and identification of bacteria infecting ticks, regardless of whether these bacteria are fastidious, obligate intracellular or noncultivable.