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Journal ArticleDOI

Ion Exchange Chromatography of Amino Acids. A Single Column, High Resolving, Fully Automatic Procedure.

P. B. Hamilton
- 01 Dec 1963 - 
- Vol. 35, Iss: 13, pp 2055-2064
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This article is published in Analytical Chemistry.The article was published on 1963-12-01. It has received 474 citations till now. The article focuses on the topics: Ion chromatography & Aqueous normal-phase chromatography.

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Citations
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Rapid analysis of amino acids using pre-column derivatization.

TL;DR: The derivatization method is rapid, efficient, sensitive, and specific for the analysis of primary and secondary amino acids in protein hydrolyzates and the liquid chromatographic system allows for the rapid, bonded-phase separation with ultraviolet detection of the common amino acids.
Journal ArticleDOI

The Chromatographic Determination of Cystine and Cysteine Residues in Proteins as S-β-(4-Pyridylethyl)cysteine

TL;DR: In order to obtain complete modification of the sulfhydryl groups in seed meals, a longer reaction time, 4.5 hours, and a concentration of 4-vinylpyridine equivalent to 3 times the mercaptoethanol concentration were required.
Journal ArticleDOI

Repair of alkylated DNA in Escherichia coli. Methyl group transfer from O6-methylguanine to a protein cysteine residue.

TL;DR: Radioactively labeled S-methylcysteine has been identified in protein hydrolysates after incubation of the alkylated DNA with a partly purified E. coli methyltransferase activity.
Journal ArticleDOI

Solubilization and Partial Purification of Tyrosine Hydroxylase from Bovine Adrenal Medulla

TL;DR: It is concluded that the previous reports that tyrosine hydroxylase is stimulated by Fe2+ can be explained by the known ability ofFe2+ to decompose H2O2.
References
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Journal ArticleDOI

A modified procedure for the automatic analysis of amino acids.

TL;DR: The procedure described allows the complete analysis of a protein hydrolyzate, including collagen, in 24 hr using a single sample, and is also suitable for many other biological amino acid mixtures.
Journal ArticleDOI

Ion Exchange Chromatography of Amino Acids

TL;DR: An apparatus for the determination of amino acids by ion exchange chromatography has a reasonable claim to being automatic, but like all other procedures thus far deit fails to go beyond analogue presentation of data.
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