Journal ArticleDOI
Isolated adrenal cells: Log dose response curves for steroidogenesis induced by ACTH1–24, ACTH1–10, ACTH4–10 and ACTH5–10
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TLDR
The isolated adrenal cell technique provides measures of both affinity and “intrinsic activity”, information of some importance to the understanding of the relation of structure to biological action among members of a series of polypeptides related to ACTH.About:
This article is published in FEBS Letters.The article was published on 1971-12-15. It has received 77 citations till now.read more
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Book ChapterDOI
Membrane receptors and hormone action.
TL;DR: This chapter describes the relation between biological activity and binding, physicochemical detection and assay of receptors, solubilization of membrane receptors, examples of membrane-localized receptors, affinity techniques, polyvalent hormone derivatives, and receptor studies, and theoretical mechanisms of hormone–receptor binding and action.
Journal ArticleDOI
Acute adrenocorticotropic hormone stimulation of adrenal corticosteroidogenesis. Discovery of a rapidly induced protein.
Rick J. Krueger,N R Orme-Johnson +1 more
TL;DR: Two-dimensional electrophoretic techniques were used to identify and characterize a protein that is not produced in quiescent isolated rat adrenal cells but is produced in response to acute stimulation by adrenocorticotropic hormone (ACTH) or dibutyryl cAMP, and are consistent with p and i being related by cotranslational modification.
Journal ArticleDOI
ACTH like peptides and opiate receptors in the rat brain. Structure-activity studies
TL;DR: The present study aims at further identifying the interaction of ACTH-like peptides and rat brain opiate receptors in vitro and finds the sequence ACTH4-10 is crucial with respect to affinity since it is the shortest sequence to inhibit the binding of [ 3H]-dihydromorphine and [3H]-naltrexone to these receptors.
Book ChapterDOI
Behavioral effects of peptides
D. de Wied,W.H. Gispen +1 more
TL;DR: The pituitary—adrenal system plays an essential role in homeostatic functions and the brain as a target for these hormones has been paid little attention.
Journal ArticleDOI
Comparative study on analgesic effect of Met5-enkephalin and related lipotropin fragments.
TL;DR: The results show that the in vivo effect is a function of the length of the peptide chain, Met5-enkephalin being the least and LPH-(61–91)-peptide the most potent.
References
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Journal ArticleDOI
Practical Procedure for Estimation of Corticosterone or Hydrocortisone
TL;DR: A simplified fluorometric procedure for corticosterone or hydrocortisone has been described and has practical applications for the estimation of these steroids in samples that are small in volume or low in concentration, or in studies that are designed primarily to demonstrate increases or decreases in adrenocortical hormones in plasma or adrenals without requiring absolute specificity.
Journal ArticleDOI
The assay of adrenocorticotrophic hormone by the adrenal ascorbic acid-depletion method.
TL;DR: The present paper is a detailed description and evaluation of the method based upon the results of a number of comparative assays carried out over the past two years, finding that the adrenocorticotrophic activity of materials other than highly purified preparations of A.C.T.H. cannot be assayed in the animal whose hypophysis has been removed.
Journal ArticleDOI
An Improved Technique for the Preparation of Isolated Rat Adrenal Cells: A Sensitive, Accurate and Specific Method for the Assay of ACTH
TL;DR: The accuracy of the isolated adrenal cell method for assay of ACTH is excellent and the cells are highly selective; they do not respond to relatively large quantities of insulin, glucagon, oxytocin, vasopressin or angiotensin II.
Journal ArticleDOI
In vitro bioassay of corticotropin: modification and statistical treatment.
Murray Saffran,A. V. Schally +1 more
TL;DR: The procedure has been modified to allow the use of standard computational methods for the calculation of the potency, and its limits, of an unknown preparation relative to a standard.
Journal ArticleDOI
A sensitive biologic assay for ACTH.
Harry S. Lipscomb,Don H. Nelson +1 more
TL;DR: The retrograde technique, while less precise, provides greater adrenal sensitivity to minute doses of ACTH than heretofore described, and is adaptable to the measurement ofACTH in biologic fluids.
Related Papers (5)
Isolated adrenal cortex cells: ACTH agonists, partial agonists, antagonists; cyclic AMP and corticosterone production
Steven Seelig,George Sayers +1 more