Journal ArticleDOI
Isolation and Callus Formation from Cotyledon Protoplasts of Pine (Pinus pinaster)
TLDR
Protoplasts were isolated from cotyledons of pine and exhibited a high frequency of spontaneous fusion, which was reduced by overnight incubation in low concentration of enzymes, which resulted in sustained division of the regenerated cells.About:
This article is published in Zeitschrift für Pflanzenphysiologie.The article was published on 1979-09-01. It has received 40 citations till now. The article focuses on the topics: Callus formation & Protoplast.read more
Citations
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Journal ArticleDOI
Somatic proembryo formation and transient expression of a luciferase gene in Douglas fir and loblolly pine protoplasts
TL;DR: Somatic pro-embryos were regenerated from morphogenic protoplasts of cell suspension cultures of Douglas fir and loblolly pine by somatic polyembryogenesis from new ESMs on agar plates and the luciferase (luc) gene was successfully introduced into fir and pine protoplast by electroporation.
Book ChapterDOI
Action of Growth Regulators
J. B. Zaerr,M. O. Mapes +1 more
TL;DR: This chapter is to summarize some of the results, particularly those from recent papers, and arrive at generalizations where possible about the mechanism of hormone action in tissue and organ culture systems used with forest species.
Journal ArticleDOI
Trends in the use of tissue culture in forest improvement
TL;DR: The analysis suggests that tissue culture will contribute significantly to the improvement of forests through exploitation of existing genotypes and production of new, commercially valuable genotypes.
Journal ArticleDOI
Somatic embryos from protoplasts of loblolly pine proembryonal cells
TL;DR: A method for regenerating somatic embryos from protoplasts derived from a cell suspension culture containing proliferating embryonal–suspensor masses (ESMs) of developing loblolly pine seeds is reported.
Journal ArticleDOI
Potential for Forest Tree Improvement via Tissue Culture
TL;DR: Considerable effort will be necessary to solve the many problems now deterring practical use of tissue culture in forest tree improvement and reforestation programs, and research must be expanded to realize the full potential available from tissue culture.
References
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Journal ArticleDOI
Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca
TL;DR: Protoplasts of soybean and N. glauca were induced to fuse with polyethylene glycol (PEG 1540) and up to 39% of the protoplasts in the treated population were heterokaryocytes, which indicated a tendency to stick together and break into pieces in the later cell generations.
BookDOI
Genetic engineering for nitrogen fixation
Alexander Hollaender,R. H. Burris,Peter Day,R. W. F. Hardy,Donald R. Helinski,M. R. Lamborg,L. Owens,R. C. Valentine +7 more
Book ChapterDOI
The Progress. Problems, and Prospects of Plant Protoplast Research
TL;DR: This chapter presents the basic information about the isolation, fusion, and culture of plant protoplasts, in pointing out some of the new research approaches that may be useful in the production of better varieties of crop plants, and in understanding the basic processes involved in the growth and differentiation of higher plants.
Book ChapterDOI
Somatic Cell Genetics and Plant Improvement
TL;DR: The chapter describes mutant isolation and selection as mutants are utilized in understanding biochemical and developmental processes in microorganisms for their potential value in plant biology.
Journal ArticleDOI
The potential of genetic manipulation in plant cell cultures for plant breeding
TL;DR: In vitro culture of somatic cells of higher plants allows the plant breeder to apply the techniques of microbial genetics to specific breeding problems in crop plants, but many techniques still require additional refinement before they will become generally applicable in a plant breeding situation.
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Somatic Polyembryogenesis from Callus of Mature Sugar Pine Embryos
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