Journal ArticleDOI
Isolation and screening of cellulolytic bacteria from soil and optimization of cellulase production and activity
TLDR
Results indicated that favorable fermentation conditions and the selection of a suitable growth medium played a key role in the production of cellulase from newly isolated Cellulomonas sp.Abstract:
Objective: The aim of the present study is to demonstrate the isolation, identification and screening of bacteria with high cellulase activity from soil samples. Materials and Methods: Cellulose degrading bacteria were isolated from soil sample using serial dilution and pour plate method. Bacteria were further identified by morphological and biochemical tests and subjected to cellulase production in 250 ml Erlenmayer flask using potato waste medium for 48h of fermentation period at 35oC with agitation speed of 140rpm. Results: Seven different bacterial strains were isolated and screened for cellulase production in submerged fermentation process. Among these seven tested bacterial strains; ASN2 showed maximum yield for cellulase production. This strain was further characterized by biochemical and morphological tests and identified as Cellulomonas sp. ASN2. Supplementation of glucose, peptone and cysteine to the fermentation medium are favored enzyme secretion. The optimum pH and temperature for the activity of crude enzyme was 7.5 and 60oC, respectively. Metal profile of the enzyme indicated that Co2+ and Mn2+ are the strong stimulators while Hg 2+ and Fe 2+ inhibited the activity of cellulase from cellulolytic bacterial strain, Cellulomonas sp.ASN2. Conclusion: Results indicated that favorable fermentation conditions and the selection of a suitable growth medium played a key role in the production of cellulase from newly isolated Cellulomonas sp. ASN2. Due to its particular characteristics this enzyme will be used in saccharification process for bioethanol production from plant biomasses.read more
Citations
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Journal ArticleDOI
Cellulase Production by Bacteria: A Review
TL;DR: The review describes the current knowledge of cellulase production by submerged fermentation and solid state fermentation, properties of cellulasing and cloning and expression of cellulases gene, and the biotechnological aspect of cellul enzyme research and their future prospects are discussed.
Journal ArticleDOI
Screening, purification and characterization of cellulase from cellulase producing bacteria in molasses.
Farjana Islam,Narayan Roy +1 more
TL;DR: According to substrate specificity, the purified cellulase had high specificity on CMC substrate which indicated it to be an endo-β-1,4-glucanase.
Journal ArticleDOI
Microbial Consortium with High Cellulolytic Activity (MCHCA) for Enhanced Biogas Production
TL;DR: The obtained results showed that the constructed MCHCA consortium is capable of efficient hydrolysis of maize silage, and increases biogas production by even 38%, depending on the inoculum used for methane fermentation.
Journal ArticleDOI
Microbial cellulolytic enzymes: diversity and biotechnology with reference to lignocellulosic biomass degradation
Santosh Thapa,Jitendra Mishra,Naveen Kumar Arora,Priya Mishra,Hui Li,Joshua O′hair,Sarabjit Bhatti,Suping Zhou +7 more
TL;DR: This review article describes the structure and functions of divergent hydrolytic enzymes (cellulolytic and xylanolytic enzymes) involved in cellulosic biomass degradation, and analyzes, outlines and unveil the prospective source of microbes encoding exceptionally diverse set of biotechnologically relevant cellulolytic enzyme sources.
Book ChapterDOI
Role of Hydrolytic Enzymes of Rhizoflora in Biocontrol of Fungal Phytopathogens: An Overview
TL;DR: This review focuses on the different aspects of various hydrolytic enzymes produced by rhizoflora and their role in sustainable biocontrol of phytopathogens.
References
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Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
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Journal ArticleDOI
Microbial cellulose utilization: fundamentals and biotechnology.
TL;DR: A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts.
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Manual For The Identification Of Medical Bacteria
TL;DR: A manual for the identification of medical bacteria is presented for the first time in a systematic fashion.
Journal ArticleDOI
Interlaboratory testing of methods for assay of xylanase activity
TL;DR: In this paper, a collaborative investigation of assays for endo-1,4-β-xylanase activity based on production of reducing sugars from polymeric 4-O-methyl glucuronoxylan was conducted.