Journal ArticleDOI
Kinetics of the mechanism of action of monoamine oxidase in the regulation of Na+,K+-ATPase activity in rat brain.
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TLDR
The removal of 3′,5′‐cyclic AMP‐dependent protein kinase from the microsomes by sodium dodecyl sulphate treatment abolished the activation and/or inhibition of the Na+,K+‐ATPase by aldehyde; it can be inferred that 3‐methoxy‐4‐hydroxybenzaldehyde‐ analogue of MAO is involved in the regulation of Na+.Abstract:
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Kinetic studies on the action of monoamine oxidase (MAO) in the regulation of Na+,K+-ATPase were performed using 3-methoxy-4-hydroxybenzaldehyde (MHB), which is an analogue of 3-methoxy-4-hydroxyphenylacetylaldehyde (product of MAO-catalysed reaction with dopamine as substrate). It was observed that at 2.6 μM MHB, the activation of Na+,K+-ATPase may be the result of the removal of the inhibitory Ca2+, thereby increasing the Vmax. Double-reciprocal plots of Pi versus MHB showed that Ca2+ counteracted the effect of the aldehyde not by changing the Km, but be decreasing the Vmax of the Na+,K+-ATPase stimulation. The removal of 3′,5′-cyclic AMP-dependent protein kinase from the microsomes by sodium dodecyl sulphate treatment abolished the activation and/or inhibition of the Na+,K+-ATPase by aldehyde; it can therefore be inferred that 3′,5′-cyclic AMP-dependent protein kinase is involved in the regulation of Na+,K+-ATPase.read more
Citations
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Journal ArticleDOI
The Na,K-ATPase of nervous tissue.
TL;DR: The Na,K-ATPase has been only partially purified from nervous tissue, yet it is clear that two forms (and ? +) of the catalytic subunit are present, which has a relatively low affinity for binding cardiac glycosides and which may also be sensitive to indirect modulation by neurotransmitters or hormones.
Journal ArticleDOI
Optic nerve regeneration in adult fish and apolipoprotein A-I.
TL;DR: The accumulation of Apo‐A‐I in nerves that are capable of regenerating may be similar to that of apo‐E in sciatic nerves of mammals (a regenerative system); in contrast, although its synthesis is increased, apo-A‐ I does not accumulate in avian optic nerves nor does apo'E in rat optic nerves (two nonregenerative systems).
Journal ArticleDOI
Low doses of vanadate and Trigonella synergistically regulate Na+/K+-ATPase activity and GLUT4 translocation in alloxan-diabetic rats
TL;DR: Combined therapy of vanadate and TSP was the most effective in normalization of altered membrane linked functions and GLUT4 distribution without any harmful side effect.
Journal ArticleDOI
Mechanism of cyclic AMP facilitation of stimulation-evoked catecholamine release in adrenal chromaffin cells—II. Inhibition of Na+,K+-ATPase by cyclic AMP
TL;DR: The ability of cyclic AMP to inhibit adrenomedullary Na + ,K + -ATPase was shown in crude membrane fraction, in leaky cell preparation and also in intact cells.
Journal ArticleDOI
Effect of acute starvation on monoamine oxidase and Na+,K(+)-ATPase activity in rat brain.
Gurcharan Kaur,Kawaljit Kaur +1 more
TL;DR: In this article, the activities of MAO and Na+, K+-ATPase were determined in rat brain after acute starvation, and they were assayed biochemically from four different regions of the brain in two subcellular fractions.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Purification and characterization of (Na+ + K+)-ATPase III. Purification from the outer medulla of mammalian kidney after selective removal of membrane components by sodium dodecylsulphate
TL;DR: Analysis of the protein composition by sodium dodecylsulphate gel electrophoresis and determination of the capacities for binding of ATP and ouabain and for sodium-dependent phosphorylation show that the procedures lead to a true purification of the enzyme.
Journal ArticleDOI
Purification and characterization of (Na+ plus K+ )-ATPase. IV. Estimation of the purity and of the molecular weight and polypeptide content per enzyme unit in preparations from the outer medulla of rabbit kidney.
TL;DR: The results suggest that the enzyme unit binding one molecule of ATP or ouabain contains two chains of large polypeptide and that both chains are phosphorylation from ATP in the presence of Mg2+ and Na+.
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