Journal ArticleDOI
Large discrete transition in a single DNA molecule appears continuous in the ensemble.
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In this paper, the authors observed giant double-stranded DNA chains by fluorescence microscopy in an aqueous environment and found that the coil-globule transition of T4DNA, 166kbp, induced by spermidine is markedly discrete for individual chains, and continuous for their ensemble average.Abstract:
We observed giant double-stranded DNA chains by fluorescence microscopy in an aqueous environment. We found that the coil-globule transition of T4DNA, 166kbp, induced by spermidine is markedly discrete for individual chains, and continuous for their ensemble average. Simple theoretical consideration is given taking account of the hierarchy of the system. It is suggested that the unique characteristics of the transition for long DNA has a general significance for the coil-globule transition in other biological and synthetic ``stiff polymers.''read more
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DNA condensation by multivalent cations.
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Christoph G. Baumann,Victor A. Bloomfield,Steven B. Smith,Carlos Bustamante,Michelle D. Wang,Steven M. Block +5 more
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TL;DR: Experimental, theoretical and numerical techniques used to examine the dynamics of biomolecules under the application of external mechanical force are discussed and certain experimental protocols are suggested to observe theoretical predictions in vitro.
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Condensed DNA: condensing the concepts.
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