Journal ArticleDOI
Lipids and calcium uptake of sperm in relation to cold shock and preservation: a review
Reads0
Chats0
TLDR
Suggestions are made for increasing the life span of sperm during preservation and microencapsulation by adding agents that may stabilize membranes, counter peroxidation and decrease calcium uptake.Abstract:
When sperm of the ram, bull, boar and stallion are cold-shocked by rapid cooling to near freezing point, motility and metabolic activity are irreversibly depressed and the acrosome and plasma membrane disrupted. Ram sperm become susceptible to cold shock in the proximal corpus region of the epididymis when the cytoplasmic droplet has moved backwards to the distal portion of the sperm midpiece. The membrane constituents phospholipids and cholesterol are important in cold shock which causes loss of lipid from sperm. The susceptibility of sperm to cold shock is linked with a high ratio of unsaturated:saturated fatty acids in the phospholipids and a low cholesterol content. The high unsaturated fatty acid content of sperm also makes them susceptible to damage from peroxidation which adversely affects motility, metabolism, ultrastructure and fertility. Hydroxynonenal, a product of fatty acid peroxidation, depresses the motility and oxygen uptake of ram sperm in vitro and may react with the -SH groups of the axonemal microtubules. High calcium concentrations in the external medium may decrease the motility and metabolism of sperm and 'calcium intoxication' may be a factor in cold shock. Lowering the environmental temperature increases calcium uptake by sperm and the effect is aggravated if the rate of cooling is rapid. Phospholipids, particularly those in egg yolk, protect sperm to some extent from cold shock and also prevent increased calcium flux into the sperm. Suggestions are made for increasing the life span of sperm during preservation and microencapsulation by adding agents that may stabilize membranes, counter peroxidation and decrease calcium uptake.read more
Citations
More filters
Journal ArticleDOI
Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon.
TL;DR: Successful semen cryopreservation enhances the advantages of AI over natural breeding, as long-term storage facilitates semen transport over distances, permits the quarantine of semen, and enables extended use of superior germplasm, even after the sire's death.
Journal ArticleDOI
Decrease in glutathione content in boar sperm after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders
Joaquín Gadea,Elena Sellés,Marco Antonio Marco,Pilar Coy,Carmen Matás,Raquel Romar,Salvador Ruiz +6 more
TL;DR: There was a loss in GSH content after cryopreservation of boar semen, and addition of GSH to the freezing extender did not result in any improvement in either standard semen parameters or sperm fertilizing ability, which suggests that during the thawing process, GSH prevents damage of a sperm property that is critical in the fertilization process but that is not measured in the routine semen analysis.
Journal ArticleDOI
Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm.
Phillip H. Purdy,James K. Graham +1 more
TL;DR: Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival.
Journal ArticleDOI
Major Proteins of Bovine Seminal Plasma Bind to the Low-Density Lipoprotein Fraction of Hen's Egg Yolk
TL;DR: It is proposed that the sequestration of BSP proteins of SP by LDF may represent the major mechanism of sperm protection by EY, and it is shown that the B SP proteins bind to the low-density fraction (LDF), a lipoprotein component of the EY extender.
Journal ArticleDOI
Membrane fluidity predicts the outcome of cryopreservation of human spermatozoa
TL;DR: It is suggested that sperm adaptability to freeze/thaw-induced stress could be dependent on their initial membrane fluidity, and this finding has practical implications for predicting the response of spermatozoa following freezing and thawing and for improving the recovery of viable spermutozoa.
References
More filters
Book
The Physiology of Reproduction
Ernst Knobil,J. D. Neill +1 more
TL;DR: The gametes, fertilization and early embryogenesis the reproductive systems - the female, the male the pituitary and the hypothalmus, and the reproductive processes and their control.
Book
The biochemistry of semen and of the male reproductive tract.
TL;DR: Bromural, Its Distribution and Decomposition In the Animal Organism, and its Differential Diagnosis from Neurological Disorders, Acta Neurol.
Journal ArticleDOI
Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa.
TL;DR: Studies with scavengers of reactive oxygen species revealed that, while reagents directed against singlet oxygen and the hydroxyl radical were without effect, cytochrome C reduced the response to A23187 by about 50%, suggesting that the superoxide anion radical is a major product of the activated human spermatozoon.