Showing papers in "Cryobiology in 2004"

TL;DR: Intracellular glasses exhibit a high molecular packing and slow molecular mobility, resembling glasses made of mixtures of proteins and sugars, which potentially interact with additional cytoplasmic components such as salts, organic acids, and amino acids above the glass transition temperature.

TL;DR: Vitrification solutions that are based on minimal perturbation of intracellular water appear to be superior and provide new hope that the successful vitrification of natural organs as well as tissue engineered or clonally produced organ and tissue replacements can be achieved.

TL;DR: There is an urgent need for the development of optimum cryopreservation methods for reproductive cells and tissues from many species, and a growing interest to understand the underlying cryobiological fundamentals responsible for these low survival rates for oocytes.

TL;DR: Although the goal of organ vitrification remains elusive, the prospects for success have never been more promising.

TL;DR: Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival.

TL;DR: It is demonstrated that lowering storage temperature progressively increases longevity of seeds, and cryogenic temperatures were not sufficient to stop deterioration, especially if initial stages of aging were allowed to progress at higher storage temperatures.

TL;DR: It is indicated that porcine oocytes at different meiotic stages respond differently to cryopreservation and MII porcINE oocytes had better resistance to cryOPreservation than GV stage oocytes.

TL;DR: The data show that RBCs can be loaded with trehalose from the extracellular medium through a combination of osmotic imbalance and the phospholipid phase transition, resulting in intracellular tre Halose concentrations of about 40 mM.

TL;DR: The proposed mechanisms provide a more comprehensive physical explanation of freezing induced cell injury and extend the understanding on solution effects.

TL;DR: Data is presented suggesting that sub-freezing temperatures, when sequentially applied with low dose chemotherapy, may provide improved cancer cell death in the freeze zone periphery, since the mechanism of action of most common chemotherapeutic agents is to initiate apoptosis in cancer cells.

TL;DR: Investigation of dimethyl sulfoxide (Me(2)SO) and glycine were investigated in an effort to improve plasma membrane integrity and mitochondrial function in cryopreserved striped bass (Morone saxatilis) sperm suggests that care must be taken to select Me( 2)SO concentration that will maximize the protection of both plasma membranes and mitochondrialfunction.

TL;DR: Array screening of hearts from control versus frozen frogs hints at the up-regulation of adenosine receptor signaling for the possible mediation of metabolic rate suppression, hypoxia inducible factor mediated adjustments of anaerobic metabolism, natriuretic peptide regulation of fluid dynamics, enhanced glucose transporter capacity for cryoprotectant accumulation, defenses against the accumulation of advanced glycation end products, and improved antioxidant defenses as novel parts of natural freeze tolerance that remain to be explored.

TL;DR: Observations of mats on the Markham Ice Shelf by high performance liquid chromatography showed that they contain high concentrations of chlorophylls a and b, and several carotenoids notably lutein, echinenone and beta-carotene, underscore the ability of microbial communities to flourish despite the severe constraints imposed by the cryo-ecosystem environment.

TL;DR: The use of sufficiently high concentrations of cryoprotectant (CPA) to vitrify rather than freeze the tissue is a possible solution to this problem, and the retention of function of embryos and elastic arteries after vitrification suggests that some cells and tissues at least can withstand exposure to the high volumes of CPA necessary for this process to occur.

TL;DR: Results demonstrated that vitrified-warmed porcine oocytes matured after IVM, developed to the blastocyst stage without distinct differences compared to fresh oocytes following ICSI and the combination of EG and Me(2)SO as a cryoprotectant was not advantageous for in vitro maturation (IVM).

TL;DR: In this paper, a comparison of the Couchman-Karasz (C-K) and Gordon-Taylor (G-T) formalisms for predicting the glass transition temperature (Tg) of a sample is presented.

TL;DR: The thermal properties of aqueous solutions with 40, 43, 45, 48, and 50% of this compound measured by differential scanning calorimetry are reported on, showing a strong decrease of averaged critical cooling and warming rates when ethanediol concentration increases.

TL;DR: The data suggest that both Bcl-2 and Bax may, not only, play an important role in the efficacy of the cryo/chemo combination, but also the balance between the two may determine the role and extent of system destruction.

TL;DR: Data are presented to show that the nucleation temperatures of a variety of solutions and freeze-avoiding insects are a function of the water volume as described by the data previously published by Bigg in 1953.

TL;DR: This work has shown that supercooled insects seem to be able to maintain their ionic gradients even at temperatures far below zero, and that ice nucleating agents may be important in this context.

TL;DR: In this paper, an intravital microscopy study was conducted to understand the flow dynamics of sinusoidal perfusion during 24-hour HMP with cold modified University of Wisconsin (UW) solution.

TL;DR: The formation of intraembryonic ice was lethal to the zebrafish embryos in all cases, but this lethal outcome was not related to solution injury effects, because 88-98% of embryos survived when exposed to a higher solute concentration with no ice present.

TL;DR: Using the condition of a constant level of supercooling of p degrees C, equations can be derived to generate non-linear cooling curves in which at all times the cooling rate is maximised, while preventing conditions that could lead to fast cooling damage.

TL;DR: Addition of sodium citrate to a sucrose solution followed by freeze-drying or convective drying resulted in a glass transition temperature (Tg) that was higher than the well-studied sucrose Tg, which concludes that citrate interacts with the sucrose OH via its carboxylate groups.

TL;DR: Findings were consistent with the hypothesis that vascular-mediated injury is responsible for defining the edge of the cryolesion in microvascular-perfused tissue, and therefore pre-induced inflammation can augment cryoinjury.

TL;DR: Using X. helleri to evaluate the effects of cryoprotectant, osmotic pressure, cooling rate, equilibration time, and sperm-to-extender ratio, sperm motility and survival duration after thawing showed significant differences.

TL;DR: The thermal properties of diethyl sulphoxide (Et2SO), as well as its cryoprotective ability are studied and related to other well-known Cryoprotectant substances, like Me2SO, and a comparison among Et2SO and other widely used cryoprotsectants has been done.

TL;DR: In conclusion, AFPs were found to protect the heart from freezing and improve survival and dP (AFP III) in prolonged subzero preservation.

TL;DR: It was found that stage III oocytes were more susceptible to chilling than stage V oocytes, and that individual female had a significant influence (p < 0.0001) on oocyte chilling sensitivity.

TL;DR: Endobronchial cryosurgery provides a safe and effective method for the palliation of otherwise inoperable lung cancer and has advantages over other methods in terms of safety, cost, and a low complication rate.