Journal ArticleDOI
Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis
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The feasibility of using PLBs for large-scale micropropagation was evaluated for scaled-up liquid cultures in bioreactors, rate of proliferation, and regeneration, and Hyponex medium was found to be suitable for conversion of PLBs into plantlets.Abstract:
Protocorm-like bodies (PLBs) formed on leaf segmentsin vitro were used as explants for bioreactor cultures. Continuous immersion cultures (air lift column and air lift-balloon bioreactor), and temporary immersion cultures (with or without charcoal filter attached) were used for the culture of PLB sections. A temporary immersion culture with charcoal filter attached was most suitable for PLB culture. About 18,000 PLBs were harvested from 20 g of inoculum (∼1000 PLB sections) in 2 l Hyponex medium after 8 weeks of incubation. Aeration in a bioreactor at 0.5 or 2.0 volume of air per volume of medium min−1 (vvm) yielded similar levels of biomass production. PLBs grown in bioreactors were cultured on solid Murashige and Skoog, Vacin and Went, Knudson C, Lindemann and Hyponex media. Hyponex medium was found to be suitable for conversion of PLBs into plantlets and 83% of PLBs transformed into plantlets on this medium. The feasibility of using PLBs for large-scale micropropagation was evaluated for scaled-up liquid cultures in bioreactors, rate of proliferation, and regeneration.read more
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Book ChapterDOI
Applications of Biotechnological Approaches in the Product and Breeding of <i>Phalaenopsis</i> Orchids
TL;DR: The role of biotechnology in the Phalaenopsis orchid industry is significant, and the development of the technology in this field will bring further benefits to researchers, producers, and fancier of Phalaena orchids as mentioned in this paper .
Pengaruh Konsentrasi Auksin dan Sitokinin terhadap Kemampuan dan Kecepatan Tumbuh Meriklon Anggrek Phalaenopsis
Eka Fibrianty,Dewi Sukma +1 more
Posted ContentDOI
Micropropagation of Brassavola nodosa (L.) Lindl. using SETIS™ bioreactor
TL;DR: In this paper , the authors evaluated the efficiency of the SETIS™ bioreactor system for the micropropagation of Brassavola nodosa by adjusting parameters of immersion (frequency and duration) and by comparing it to conventional semi-solid culture systems.
Universidade federal de santa catarina centro de ciências agrárias programa de pós-graduação em recursos genéticos vegetais
TL;DR: This work focuses on Cattleya amethystoglossa Linden & Rchb.
Journal ArticleDOI
The Establishment of Rapid Propagation System of ‘RED SUN’ Phalaenopsis aphrodite
TL;DR: The best combination of initiation culture was collecting peduncles in January + upper position of peduncle + 0.1% HgCl2 sterilization for 8 min this paper .
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
Some pH Changes in Nutrient Solutions
Emil F. Vacin,F. W. Went +1 more
TL;DR: An investigation indicated that amino acids are excellent buffers and a new nutrient solution is proposed, based on the results of studies on weakly buffered culture media used for asymbiotic germination of orchid seeds.
Book
Micropropagation of Orchids
Joseph Arditti,Robert Ernst +1 more
TL;DR: This book discusses the origins of Orchid Micropropagation, its applications, and some of the sources of Supplies, Equipment, Terms, and Reagents, as well as methods for specific Genera for Specific Genera.
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A revised medium for rapid growth and bio assays with tobacco tissue cultures
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