Journal ArticleDOI
Mode of action of the cytostatic agent "ICRF 159".
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‘ICRF 159’, a new cytostatic agent, blocks the entry of cultured human lymphocytes into mitosis and arrests dividing cells in prophase and early metaphase.Abstract:
‘ICRF 159’, a new cytostatic agent, blocks the entry of cultured human lymphocytes into mitosis and arrests dividing cells in prophase and early metaphase.read more
Citations
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Antitumor bisdioxopiperazines inhibit yeast DNA topoisomerase II by trapping the enzyme in the form of a closed protein clamp.
TL;DR: Inhibition of interconversion between the open- and closed-clamp forms of type II DNA topoisomerases offers a new mechanism in the selection and design of therapeutics targeting this class of enzymes.
Journal ArticleDOI
A topoisomerase II-dependent G2 cycle checkpoint in mammalian cells
C. Stephen Downes,Duncan J. Clarke,Ann M. Mullinger,Juan F. Giménez-Abián,Andrew M. Creighton,Robert T. Johnson +5 more
TL;DR: It is found that exit from G2 is regulated by a catenation-sensitive checkpoint mechanism which is distinct from the G2-damage checkpoint, indicating that topoisomerase II is needed for complete condensation.
Journal ArticleDOI
Catalytic inhibitors of DNA topoisomerase II
Toshiwo Andoh,Ryoji Ishida +1 more
TL;DR: Bis(2,6-dioxopiperazines) have a potential to overcome cardiac toxicity caused by potent antitumor anthracycline antibiotics such as doxorubicin and daunorubsicin, and enhance the efficacy of topoisomerase II poisons by reducing their side effects in preclinical and clinical settings.
Journal ArticleDOI
The Potential of Iron Chelators of the Pyridoxal Isonicotinoyl Hydrazone Class as Effective Antiproliferative Agents II: The Mechanism of Action of Ligands Derived From Salicylaldehyde Benzoyl Hydrazone and 2-Hydroxy-1-Naphthylaldehyde Benzoyl Hydrazone
TL;DR: Investigation of the mechanism of action of these PIH analogues in a variety of neoplastic cell lines showed that the analogues were far more active than DFO at inhibiting cellular proliferation and 3H-thymidine, 3h-leucine, and3H-uridine incorporation, and provided further evidence for the potential use of these Fe chelators as anticancer agents.
Journal ArticleDOI
Inhibition of DNA topoisomerase II by ICRF-193 induces polyploidization by uncoupling chromosome dynamics from other cell cycle events.
TL;DR: The results strongly suggest that the essential roles of topo II are confined to the M phase, when the enzyme decatenates intertwined replicated chromosomes, in other phases of the cycle, including the S phase.
References
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Journal ArticleDOI
Chromosome preparations of leukocytes cultured from human peripheral blood
TL;DR: A combination of cytological and leukocyte culture techniques is described which constitutes a convenient, reliable approach for chromosome studies of humans and yields the following advantages: relative ease of obtaining blood and small volume required.
Journal ArticleDOI
DNA synthesis and mitosis in cultures of human peripheral leukocytes.
M.A. Bender,D.M. Prescott +1 more
TL;DR: DNA synthesis and mitosis in human peripheral blood leukocyte cultures have been studied using tritiated thymidine using Phytohemagglutinin to induce a very great increase in the level of DNA synthesis andMitosis.
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Antitumour activity in a series of bisdiketopiperazines.
TL;DR: The idea of attempting to influence the growth of cells and tumours with chelating agents has been examined because many enzyme systems depend on or contain trace metals.
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Preliminary clinical assessment of I.C.R.F. 159 in acute leukaemia and lymphosarcoma.
TL;DR: I.R.C.F. 159, a new antitumour agent, has been assessed in patients with acute leukaemia and three with lymphosarcoma; in all but two there was a considerable fall in circulating primitive cells, and in one there was bone-marrow evidence of a partial remission.