Modifier effects on packed and capillary columns in supercritical fluid chromatography

TLDR: In this article, the chromatographic effects of different concentrations of an additive, isopropanol, in carbon dioxide have been studied on capillary and packed columns, and the best results were obtained with carbon dioxide/8% isOPropanol as mobile phase on a capillary column coated with a cyanopropyl-substituted polysiloxane stationary phase.

Abstract: The separation of polar thermally labile solutes is one of the potentially most rewarding fields of SFC application. A presupposition for such applications is, however, mobile phases having relatively high solvent strengths. A promising approach to achieve this is the use of mobile phases consisting of carbon dioxide with a polar additive. In this work, the chromatographic effects of different concentrations of an additive, isopropanol, in carbon dioxide have been studied on capillary and packed columns. A series of antibiotics was used as test substances. Best results were obtained with carbon dioxide/8% isopropanol as mobile phase on a capillary column coated with a cyanopropyl-substituted polysiloxane stationary phase.