Molecular typing of Treponema pallidum strains from patients with neurosyphilis in Pretoria, South Africa.
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TLDR
The molecular typing system for Treponema pallidum using cerebrospinal fluid specimens obtained from patients with neurosyphilis in Pretoria, South Africa shows that the typing system can be applied to specimens which may contain low numbers of spirochaetes such as CSF.Abstract:
Objective: To evaluate the molecular typing
system for Treponema pallidum using cerebrospinal fluid
specimens obtained from neurosyphilis patients in
Pretoria, South Africa. Methods: CSF specimens were collected from 32 men
and 18 women with suspected late neurosyphilis. Typing
of T. pallidum involved PCR amplification and
restriction analysis of the tpr E, G, and J genes and
determination of the number of 60-bp tandem repeats
within the arp gene by PCR amplification.
Results: Of 13 typeable specimens, 4 strain types
were identified: 2i, 3e, 14a, and 17e. Subtype 14a was
identified in 7 specimens (53.8%) whilst 4 specimens
(30.7%) were subtype 3e, and 1 specimen (7.6%) each had
subtype 17e and 2i. Conclusions: This study shows that the typing
system can be applied to specimens which may contain low
numbers of spirochetes such as cerebrospinal fluid.read more
Citations
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Syphilis: using modern approaches to understand an old disease
Emily L. Ho,Sheila A. Lukehart +1 more
TL;DR: Recent progress in the application of modern molecular techniques to understanding the biological basis of this multistage disease and to the development of new tools for diagnosis, for predicting efficacy of treatment with alternative antibiotics, and for studying the transmission of infection through population networks are reviewed.
Journal ArticleDOI
Enhanced Molecular Typing of Treponema pallidum: Geographical Distribution of Strain Types and Association with Neurosyphilis
Christina M. Marra,Sharon K. Sahi,Lauren C. Tantalo,Charmie Godornes,Tara B. Reid,Frieda Behets,Anne Rompalo,Jeffrey D. Klausner,Yue Ping Yin,Fiona Mulcahy,Matthew R. Golden,Matthew R. Golden,Arturo Centurion-Lara,Sheila A. Lukehart +13 more
TL;DR: An enhanced T. pallidum strain typing system that shows biological and clinical relevance is described and is believed to be the most discriminating typing system.
Journal ArticleDOI
Macrolide Resistance and Molecular Types of Treponema pallidum Causing Primary Syphilis in Shanghai, China
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Genetic diversity in Treponema pallidum: implications for pathogenesis, evolution and molecular diagnostics of syphilis and yaws.
TL;DR: Genome analyses of pathogenic uncultivable treponemes revealed striking similarity among these bacteria and also a high degree of similarity to the rabbit pathogen, Treponema paraluiscuniculi, a trePoneme not infectious to humans.
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Molecular Characterization of Syphilis in Patients in Canada: Azithromycin Resistance and Detection of Treponema pallidum DNA in Whole-Blood Samples versus Ulcerative Swabs
Irene Martin,Raymond S. W. Tsang,Karen Sutherland,Peter A. G. Tilley,Ron Read,Barbara Anderson,Colleen Roy,Ameeta E. Singh +7 more
TL;DR: The results confirmed that swabs from primary ulcers are the specimens of choice for laboratory diagnostic purposes, however, further research is required to determine what specimen(s) would be most appropriate for molecular investigation of syphilis in secondary and latent syphilis.
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