Open AccessJournal Article
Monoclonal antibodies (F36/22 and M7/105) to human breast carcinoma.
Lawrence D. Papsidero,Gary A. Croghan,Mary Jane O'Connell,Luis A. Valenzuela,Takuma Nemoto,T. Ming Chu +5 more
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TLDR
Cloned hybridoma cell lines obtained from fusions of murine myeloma cells with lymphocytes of mice immunized against human breast cancer cells indicated that antibodies F36/22 and M7/105 recognized determinants expressed maximally on Breast cancer cells.Abstract:
Cloned hybridoma cell lines were obtained from fusions of murine myeloma cells with lymphocytes of mice immunized against human breast cancer cells. Hybridomas F36/22 and M7/105 produced antibodies whose binding to breast cancer cells could not be inhibited by prior absorptions with fibroblasts, lymphoblastoid cells, or erythrocytes. Results from cell surface binding assays using a panel of tumor cell lines indicated that antibodies F36/22 and M7/105 recognized determinants expressed maximally on breast cancer cells. Antibody F36/22 reacted with normal mammary epithelial membranes and milk fat globule membranes, whereas antibody M7/105 produced no detectable binding to these specimens. Antigens carrying these epitopes each showed reactivity with concanavalin A lectin. The determinant corresponding to antibody F36/22 was detectable in histological sections of a subset of breast tumors obtained at surgery.read more
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Aberrant glycosylation in tumors and tumor-associated carbohydrate antigens
TL;DR: Aberrant glycosylation as such may be the basis of inappropriate cell/cell and cell/matrix interactions that may be reflected in the abnormal cell social behavior of tumor cells, such as uncontrolled cell growth, invasiveness, and metastatic potential.
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Differential reactivity of a novel monoclonal antibody (DF3) with human malignant versus benign breast tumors.
TL;DR: Results demonstrate that the DF3 antigen is present on apical borders of more differentiated secretory mammary epithelial cells and in the cytosol of less differentiated cells.
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Monoclonal antibodies against human milk‐fat globule membranes detecting differentiation antigens of the mammary gland and its tumors
John Hilkens,F. Buijs,Jo Hilgers,Philomena C. Hageman,Jero Calafat,Arnoud Sonnenberg,van der Valk M +6 more
TL;DR: The specificity of the antibodies, tested individually, was not sufficient for further differential diagnosis of the carcinomas, but when some of these antibodies were used in a panel they contribute to an important improvement of the diagnosis.
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Distribution of Oncofetal Antigen Tumor-associated Glycoprotein-72 Defined by Monoclonal Antibody B72.3
TL;DR: The pancarcinoma distribution and lack of significant reactivity with normal adult tissues of monoclonal antibody B72.3 suggest its potential diagnostic and therapeutic utility for human carcinomas.
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Use of a murine monoclonal antibody for detection of circulating plasma DF3 antigen levels in breast cancer patients.
TL;DR: The results suggest that DF3 antigen levels may be useful in distinguishing breast cancer patients from those with esophageal, gastric, colorectal, pancreatic, and lung carcinomas, as well as following the clinical course of patients with metastatic breast cancer.
References
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Journal ArticleDOI
Continuous cultures of fused cells secreting antibody of predefined specificity
G Köhler,C. Milstein +1 more
TL;DR: The derivation of a number of tissue culture cell lines which secrete anti-sheep red blood cell (SRBC) antibodies is described here, made by fusion of a mouse myeloma and mouse spleen cells from an immunised donor.
Journal ArticleDOI
A Human Cell Line From a Pleural Effusion Derived From a Breast Carcinoma
Book ChapterDOI
New Human Tumor Cell Lines
Jørgen Fogh,Germain Trempe +1 more
TL;DR: The careful attention to all the minute details provided by the dedicated tissue culturist seems to be the most important of all factors and cannot well be substituted for by special recipes or fancy equipment.
Journal ArticleDOI
Colorectal carcinoma-specific antigen: detection by means of monoclonal antibodies
TL;DR: Two hybridomas and their clones secreted antibodies binding specifically to human colorectal carcinoma cells either grown in culture or obtained from patients, but did not bind to normal colonic mucosa or other normal and malignant human cells.
Journal ArticleDOI
A spectrum of monoclonal antibodies reactive with human mammary tumor cells
TL;DR: Eleven monoclonal antibodies were chosen that demonstrated reactivities with human mammary tumor cells and not with apparently normal human tissues, and all five major groups demonstrated binding to human metastatic mammary carcinoma cells both in axillary lymph nodes and at distal sites.