Journal ArticleDOI
Morphologic criteria and detection of apoptosis.
TLDR
Increasing evidence shows that TUNEL-positive cardiomyocytes and internucleosomal DNA fragmentation are associated with various cardiac diseases, including acute myocardial infarction and heart failure.Abstract:
Apoptosis is an organized, energy dependent process, which leads to cell death. Its definition is based on distinct morphological features [10] and demonstration of internucleosomal DNA degradation [27], executed by selectively activated DNAses [4, 22]. The morphologic hallmarks of apoptosis include chromatic margination, nuclear condensation and fragmentation, and condensation of the cell with preservation of organelles. The process is followed by fragmentation of the cell into membrane-bound apoptotic bodies, which undergo phagocytosis by nearby cells without associated inflammation [10, 11]. Apoptosis characteristically occurs in isolated single cells. The duration of apoptosis is estimated to be from 12 to 24 hours, but in cell culture visible morphologic changes are accomplished in less than two hours [10, 16]. Non-apoptotic cell death, a prototype of which is cell death due to ischemia (oncosis), is characterized by depletion of intracellular ATP stores, swelling of the cell with disruption of organelles and rupture of the plasma membrane [15]. Groups of necrotic cells and inflammation are found in tissues [10, 15].read more
Citations
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Journal ArticleDOI
Morphologic and biochemical hallmarks of apoptosis.
Antti Saraste,Kari Pulkki +1 more
TL;DR: A review of the molecular mechanisms of apoptosis as they relate to the morphologic hallmarks and their implications for the detection of cancer cell apoptosis in cardiac tissue is presented in this article.
Journal Article
Acute doxorubicin cardiotoxicity involves cardiomyocyte apoptosis.
Olli Arola,Antti Saraste,Kari Pulkki,Markku Kallajoki,Martti Parvinen,Liisa-Maria Voipio-Pulkki +5 more
TL;DR: Doxorubicin-induced cardiotoxicity involves cardiomyocyte apoptosis, a potentially preventable form of myocardial tissue loss, and alterations in the cytoskeletal apparatus with focal loss of contractile elements were seen after a single injection.
Journal ArticleDOI
Electron microscopy of oligodendroglia in severe mental illness.
Natalya A. Uranova,D.D. Orlovskaya,O.V. Vikhreva,I S Zimina,Natalya S. Kolomeets,Victor M. Vostrikov,V.I. Rachmanova +6 more
TL;DR: Qualitative electron microscopy was performed to verify whether brain pathology in schizophrenia and bipolar disorder is associated with alterations of oligodendroglial cells and myelinated fibers and demonstrated that changes could not be explained by the effects of postmortem delay, age, neuroleptic medication, or gender.
Journal ArticleDOI
Apoptosis in Parkinson's disease: Signals for neuronal degradation
TL;DR: A review of the molecular events that underlie apoptotic nuclear degradation and the literature regarding the unreliability of 3' DNA end labeling as a marker of apoptotic degradation is presented in this article.
Book ChapterDOI
Detection of apoptosis by TUNEL assay.
TL;DR: An assay for detection of apoptotic cells during mouse odontogenesis using a colorimetric TUNEL system is described using the ability of TdT to label blunt ends of double-stranded DNA breaks independent of a template.
References
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Journal ArticleDOI
Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.
TL;DR: Apoptosis seems to be involved in cell turnover in many healthy adult tissues and is responsible for focal elimination of cells during normal embryonic development, and participates in at least some types of therapeutically induced tumour regression.
Journal ArticleDOI
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
TL;DR: The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.
Journal ArticleDOI
Caspases: Enemies Within
TL;DR: This work has shown that understanding caspase regulation is intimately linked to the ability to rationally manipulate apoptosis for therapeutic gain.
Journal ArticleDOI
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
TL;DR: It is shown here that this morphological change is closely associated with excision of nucleosome chains from nuclear chromatin, apparently through activation of an intracellular, but non-lysosomal, endonuclease.
Journal Article
Apoptosis, oncosis, and necrosis : an overview of cell death
Guido Majno,Isabelle Joris +1 more
TL;DR: Some of the typical features of apoptosis are discussed, such as budding (as opposed to blebbing and zeiosis) and the inflammatory response, and stands in contrast to apoptosis, which leads to necrosis with karyorhexis and cell shrinkage.