Reperfusion injury induces apoptosis in rabbit cardiomyocytes.
Roberta A. Gottlieb,Katharine O. Burleson,Robert A. Kloner,Bernard M. Babior,Robert L. Engler +4 more
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Parts of apoptosis (programmed cell death) in myocytes are identified as a response to reperfusion but not ischemia, which indicates that apoptosis may be a specific feature of reperfusions injury in cardiac myocytes, leading to late cell death.Abstract:
The most effective way to limit myocardial ischemic necrosis is reperfusion, but reperfusion itself may result in tissue injury, which has been difficult to separate from ischemic injury. This report identifies elements of apoptosis (programmed cell death) in myocytes as a response to reperfusion but not ischemia. The hallmark of apoptosis, nucleosomal ladders of DNA fragments (approximately 200 base pairs), was detected in ischemic/reperfused rabbit myocardial tissue but not in normal or ischemic-only rabbit hearts. Granulocytopenia did not prevent nucleosomal DNA cleavage. In situ nick end labeling demonstrated DNA fragmentation predominantly in myocytes. The pattern of nuclear chromatin condensation was distinctly different in reperfused than in persistently ischemic tissue by transmission electron microscopy. Apoptosis may be a specific feature of reperfusion injury in cardiac myocytes, leading to late cell death.read more
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Journal ArticleDOI
Apoptosis in the pathogenesis and treatment of disease
TL;DR: In multicellular organisms, homeostasis is maintained through a balance between cell proliferation and cell death, and recent evidence suggests that alterations in cell survival contribute to the pathogenesis of a number of human diseases.
Journal ArticleDOI
Apoptosis in the Failing Human Heart
Giorgio Olivetti,R Abbi,Federico Quaini,Jan Kajstura,Cheng W,James A. Nitahara,Eugenio Quaini,C. Di Loreto,Carlo Alberto Beltrami,Stanislaw Krajewski,John C. Reed,Piero Anversa +11 more
TL;DR: Programmed death of myocytes occurs in the decompensated human heart in spite of the enhanced expression of BCL2; this phenomenon may contribute to the progression of cardiac dysfunction.
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Molecular Mechanisms of Myocardial Remodeling
TL;DR: This review focuses only on permanent modifications in relation to clinical dysfunction in cardiac remodeling secondary to myocardial infarction and/or arterial hypertension and includes a special section on the senescent heart, since CR is mainly a disease of the elderly.
Journal ArticleDOI
Apoptosis in Myocytes in End-Stage Heart Failure
Jagat Narula,Neena B. Haider,Renu Virmani,Thomas G. DiSalvo,Frank D. Kolodgie,Roger J. Hajjar,U Schmidt,Marc J. Semigran,G W Dec,Ban-An Khaw +9 more
TL;DR: Loss of myocytes due to apoptosis occurs in patients with end-stage cardiomyopathy and may contribute to progressive myocardial dysfunction.
Journal ArticleDOI
Morphologic and biochemical hallmarks of apoptosis.
Antti Saraste,Kari Pulkki +1 more
TL;DR: A review of the molecular mechanisms of apoptosis as they relate to the morphologic hallmarks and their implications for the detection of cancer cell apoptosis in cardiac tissue is presented in this article.
References
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Journal ArticleDOI
Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics.
TL;DR: Apoptosis seems to be involved in cell turnover in many healthy adult tissues and is responsible for focal elimination of cells during normal embryonic development, and participates in at least some types of therapeutically induced tumour regression.
Journal ArticleDOI
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation.
TL;DR: The extent of tissue-PCD revealed by this method is considerably greater than apoptosis detected by nuclear morphology, and thus opens the way for a variety of studies.
Book ChapterDOI
Cell death : the significance of apoptosis
TL;DR: It has proved feasible to categorize most if not all dying cells into one or the other of two discrete and distinctive patterns of morphological change, which have, generally, been found to occur under disparate but individually characteristic circumstances.
Journal ArticleDOI
Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation
TL;DR: It is shown here that this morphological change is closely associated with excision of nucleosome chains from nuclear chromatin, apparently through activation of an intracellular, but non-lysosomal, endonuclease.
Journal ArticleDOI
Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis.
TL;DR: The data confirm that the condensed chromatin which characterizes apoptosis morphologically consists of endogenously digested chromatin fragments, and provide support for the view that at least some cells enter apoptosis by a process dependent upon macromolecular synthesis.