Journal ArticleDOI
Multichannel Confocal Microscope Based on a Diffraction Focusing Multiplier with Automatic Synchronization of Scanning
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TLDR
In this paper, an implementation of a laser scanning confocal microscope is described, where the specimen is scanned by an array of illuminating beams, which significantly increases the velocity of object image construction.Abstract:
Implementation of a laser scanning confocal microscope is described, where the specimen is scanned by an array of illuminating beams, which significantly increases the velocity of object image construction. The array formation is provided by using a diffractive optical element. Scanning by the array of laser beams over the specimen is performed by galvanometric scanners with moving refractive plane-parallel plates. Owing to application of such a scanning device, the beams in the illuminating channel and the signal beams in the receiving channel pass through one motionless array of confocal diaphragms; as a result, the scanning beams in the specimen plane and the signal beams in the plane of the photodetector matrix can be used without an additional synchronized pair of scanners. The proposed confocal microscope can be applied in problems that require a fast response.read more
Citations
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Journal ArticleDOI
Development of the Spaser-in-Liposome Complexes for Theranostical Application
TL;DR: In this article, the possibility of forming a complex of spasers inside artificially created closed membranes (liposomes) was studied experimentally and the localization of the complexes inside liposomes was confirmed using a scanning confocal microscope.
References
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Book
Handbook of biological confocal microscopy
TL;DR: The third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging as mentioned in this paper.
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High-speed 1-frame/ms scanning confocal microscope with a microlens and Nipkow disks.
Takeo Tanaami,Shinya Otsuki,Tomosada Nobuhiro,Kosugi Yasuhito,Mizuho Shimizu,Hideyuki Ishida +5 more
TL;DR: A high-speed confocal laser microscope developed with a microlens- array disk set in front of a pinhole-array disk improved optical efficiency more than ten times compared with that of conventional Nipkow confocal microscopy.
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Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging
Togo Shimozawa,Kazuo Yamagata,Takefumi Kondo,Shigeo Hayashi,Atsunori Shitamukai,Daijiro Konno,Fumio Matsuzaki,Jun Takayama,Shuichi Onami,Hiroshi Nakayama,Yasuhito Kosugi,Tomonobu M. Watanabe,Katsumasa Fujita,Yuko Mimori-Kiyosue +13 more
TL;DR: Spinning disk confocal microscopy is improved by eliminating pinhole cross-talk and the generation of out-of-focus light is prevented by two-photon excitation that achieves selective-plane illumination, dramatically improving higher-resolution intravital imaging.
Journal ArticleDOI
High-speed surface measurement using a nonscanning multiple-beam confocal microscope
Mitsuhiro Ishihara,Hiromi Sasaki +1 more
TL;DR: In this paper, a nonscanning multiple-beam confocal microscope is proposed for in-line semiconductor package inspections. But the system is not suitable for high-speed surface measurement.