Open AccessBook
Handbook of biological confocal microscopy
Reads0
Chats0
TLDR
The third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging as mentioned in this paper.Abstract:
This third edition of a classic text in biological microscopy includes detailed descriptions and in-depth comparisons of parts of the microscope itself, digital aspects of data acquisition and properties of fluorescent dyes, the techniques of 3D specimen preparation and the fundamental limitations, and practical complexities of quantitative confocal fluorescence imaging.read more
Citations
More filters
Journal ArticleDOI
A guided tour into subcellular colocalization analysis in light microscopy
TL;DR: A novel toolbox for subcellular colocalization analysis under ImageJ is created that integrates current global statistic methods and a novel object‐based approach to assess proteins residing on intracellular structures by fluorescence microscopy.
Journal ArticleDOI
Ultra-High Resolution Imaging by Fluorescence Photoactivation Localization Microscopy
TL;DR: A new method for fluorescence imaging has been developed that can obtain spatial distributions of large numbers of fluorescent molecules on length scales shorter than the classical diffraction limit, and suggests a means to address a significant number of biological questions that had previously been limited by microscope resolution.
Journal ArticleDOI
Far-Field Optical Nanoscopy
TL;DR: Initial applications indicate that emergent far-field optical nanoscopy will have a strong impact in the life sciences and in other areas benefiting from nanoscale visualization.
Journal ArticleDOI
Super-Resolution Fluorescence Microscopy
TL;DR: It is anticipated that super-resolution fluorescence microscopy will become a widely used tool for cell and tissue imaging to provide previously unobserved details of biological structures and processes.
Journal ArticleDOI
Three-Dimensional Resolution Doubling in Wide-Field Fluorescence Microscopy by Structured Illumination
Mats G. L. Gustafsson,Lin Shao,Peter M. Carlton,C. J. Rachel Wang,Inna N. Golubovskaya,W. Zacheus Cande,David A. Agard,David A. Agard,John W. Sedat +8 more
TL;DR: This work describes how spatially structured illumination microscopy can be applied in three dimensions to double the axial as well as the lateral resolution, with true optical sectioning, and has produced the first light microscopy images of the synaptonemal complex in which the lateral elements are clearly resolved.
Related Papers (5)
Two-Photon Laser Scanning Fluorescence Microscopy
Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion fluorescence microscopy
Stefan W. Hell,Jan Wichmann +1 more