Journal ArticleDOI
Notizen: A Rapid Visualization of Kleinschmidt-Type DNA Preparations by Phosphotungstic Acid
Michiko Egel-Mitani,Richard Egel +1 more
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This article is published in Zeitschrift für Naturforschung B.The article was published on 1972-04-01. It has received 9 citations till now. The article focuses on the topics: Phosphotungstic acid.read more
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DNA electron microscopy.
TL;DR: The most important methods are based on nucleic acid hybridization: DNA-DNA hybridization (heteroduplex, D-loop), RNA-DNA (Rloop), or combinations of both (R-hybrid), which allow both qualitative and quantitative analysis of gene organization, position and extension of homology regions, and characterization of transcription.
Journal ArticleDOI
Nuclear membranes from mammalian liver. IV. Characterization of membrane-attached DNA.
Werner W. Franke,Barbara Deumling,Barbara Deumling,Barbara Deumling,Hanswalter Zentgraf,Hanswalter Zentgraf,Hanswalter Zentgraf,Heinz Falk,Heinz Falk,Heinz Falk,P. M.M. Raex,P. M.M. Raex,P. M.M. Raex +12 more
TL;DR: The results reflect the close relationship between the nuclear envelope and (constitutive) heterochromatin, but also indicate that membrane binding is not restricted to this material.
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Nuclear membranes from mammalian liver: VI. Glucose-6-phosphatase in rat liver, a cytochemical and biochemical study
TL;DR: The results clarify the divergence of earlier reports concerning the presence of G-6-Pase in the perinuclear cisterna and add biochemical evidence to the morphologically derived view of the nuclear envelope as being a special form of the ER system.
Journal ArticleDOI
Alkaline lysis of mammalian cells for sedimentation analysis of nuclear DNA. Conformation of released DNA as monitored by physical, electron microscopic and enzymological techniques.
Silvio Parodi,Richard A. Mulivor,Joseph T. Martin,Claudio Nicolini,D.S.R. Sarma,Emanuel Farber +5 more
TL;DR: The degree of single strandedness of the DNA released from rat liver nuclei by various alkaline lysing solutions was determined both before and after sedimentation in alkaline sucrose gradients employing electron microscopy, melting profiles, circular dichroism measurements, and digestibility by S1 nuclease.
Journal ArticleDOI
Transcriptional role in DNA replication: Degradation of RNA primer during DNA synthesis
Ranajit Roychoudhury,Hans Kössel +1 more
TL;DR: The fragment of DNA polymerase lacking the 5′→3′ exonuclease shows comparable RNA primer dependency but reduced efficiency for the degradation of the RNA primer from the5′-end.