Showing papers in "Biochemical and Biophysical Research Communications in 1973"

TL;DR: Evidence is presented that indicates a regular sub-structure in nuclear nucleoprotein with regularly distributed sites that are specifically susceptible to the cellular Ca-Mg endonuclease.

TL;DR: A simple method is reported to detect cooperative interactions in the binding of polypeptide hormones to their membrane receptors, and Insulin receptors on cultured lymphocytes and liver plasma membranes show negative cooperative interactions.

TL;DR: The results suggest that hydroxylated proline increases the thermal stability of collagen.

TL;DR: These results are interpreted to indicate the localization of nearly all of the phosphatidylserine on the interior surface of the membrane thus demonstrating an asymmetric distribution of phospholipids in the erythrocyte membrane.

TL;DR: 3-Hydroxy-3-methylglutaryl coenzyme A reductase activity is diminished in several in vitro liver systems preincubated in the presence of cAMP, and can be partially restored by incubation with a second protein fraction of the cytosol.

TL;DR: The phase diagrams of binary mixtures of cholesterol and dipalmitoyl phosphatidylcholine, and cholesterol and dimyristoyl phosphate in the presence of excess water have been investigated using spin labels usingspin labels.

TL;DR: A simple and rapid procedure is described for the reconstitution of biologically active phospholipid vesicles without the use of detergents and the following systems have been reconstituted by this procedure.

TL;DR: A mutant strain of Beijerinckia B8/36 oxidizes biphenyl to cis -2, 3-dihydroxy-1-phenylcyclohexa-4, 6-diene, and the physical properties of both metabolites are described.

TL;DR: It is suggested that the peroxidative oxidation of unsaturated lipid promoted by xanthine oxidase involves the formation of singinglet oxygen from superoxide, and the singlet oxygen reacts with the lipid to form fatty acid hydroperoxides.

TL;DR: The primary structure of this peptide, named somatostatin, has been determined to be H-Ala-Gly- Cys-Lys-Asn-Phe- Phe-Trp-LYS-Thr-P he-ThR-Ser-Cys -OH, which was confirmed by direct mass spectrometry.

TL;DR: This finding suggests that the presence of hydroxyproline in the molecule contributes significantly to the thermal stability of collagen, and suggests that triple strand formation may be required for normal secretion.

TL;DR: Evidence is presented that indicates that microsomal cytochrome P-450 acts as the catalyst and only aminopyrine and dimethylaniline are rapidly oxidized by hydroperoxides in the presence of catalase.

TL;DR: Heretofore unrecognized metabolites of methotrexate (MTX) have been detected in human red blood cells and isolated from rat liver and viscera by comparison with authentic synthetic compounds.

TL;DR: The results demonstrate the biological role and importance of superoxide dismutase and the role of ribonuclease and lysine tRNA ligase in protecting against superoxide radicals in various biological systems.

TL;DR: The superoxide dismutase from bovine erythrocytes has been shown to catalyze the reduction of oxygen by H2O2, and the reversal of the spontaneous disproportionation of superoxide radicals was made feasible through the use of tetranitromethane as an effective scavenger for thesuperoxide radicals.

TL;DR: The effect of two membrane-active antimicrobials; cetrimide upon E. coli and amphotericin upon C.albicans is related to the leakage of phosphate and 260 nm material.

TL;DR: The binding of a series of proteins to agarose substituted with n-alkylamines of varying C-chain length appears to depend on cooperation between hydrophobic and electrostatic forces, the latter involving positive charges on the adsorbent which are introduced by the substitution process.

TL;DR: 5S RNA is most likely part of a site with which the TψC-loop of tRNA interacts on the ribosome, and could be abolished by selective chemical modification of two adenines in 5S RNA.

TL;DR: Phosphorylated non-histone chromatin proteins are shown to stimulate the synthesis of RNA in a cell-free system using rat liver RNA polymerase and rat DNA as template.

TL;DR: Liver microsomes and partially purified cytochromes P450 prepared from untreated animals or those injected with phenobarbital, or 3-methylcholanthrene, or polychlorinated biphenyls, were subjected to slab-gel SDS-electrophoresis.

TL;DR: In this article, the authors found that the growth behavior of NIL cells in medium containing globoside changes due to an extended prereplicative phase and a reduced saturation density, which may result from changed adhesive properties of cells.

TL;DR: The data presented support a role for the catenated DNA molecule as an intermediate in the replication of the plasmid R6K.

TL;DR: The increase in reductase activity in rat liver increased 2 to 7-fold after subcutaneous administration of insulin into normal or diabetic animals and was accompanied by a proportionate increase in activity for the conversion of acetate to cholesterol.

TL;DR: The dicoumarol-induced prothrombin had only one high affinity binding site and no ligand-induced conformational change was detected in this prothromabin.

TL;DR: It is proposed that previous conflicts regarding the molecular weight of this glycoprotein can be resolved by reference to the isolation and/or solubilization conditions.

TL;DR: Fortified hamster and rat liver microsomes bind the carcinogen aflatoxin B1, but not its much less carcinogenic 2,3-dihydro derivative (aflatoxin B2), to RNA in vitro, and this epoxide merits consideration as a candidate ultimate carcinogenic metabolite of AFB1.

TL;DR: FL-1060, in contrast to other penicillins, does not inhibit murein transpeptid enzyme, D-alanine carboxypeptidase I, or mureIn endopeptidases.

TL;DR: The fluorescamine procedure is now applicable to the full array of natural amino acids, and provides an efficient fluorometric assay for secondary amino acids.

TL;DR: An endonuclease has been identified which requires both calcium and magnesium ions for activity and which is responsible for the calcium primed DNA synthesis observed in certain nuclear preparations.

TL;DR: In 0.01 M Tris-HCl buffer, pH 8, a number of arbitrarily chosen enzymes with isoelectric points in the acid region (xanthine oxidase, lactate dehydrogenase, DNase I, alkaline phosphatase and urease) are virtually irreversibly bound by mere adsorption to agarose substituted with either n-butylamine or n-octylamine.