Showing papers in "Biochimica et Biophysica Acta in 1975"

TL;DR: Although empirical predictions based on larger numbers of known protein structure tend to be more accurate than those based on a limited sample, the improvement in accuracy is not dramatic, suggesting that the accuracy of current empirical predictive methods will not be substantially increased simply by the inclusion of more data from additional protein structure determinations.

TL;DR: This review focuses on work that deals with the mechanisms of detergent action in membrane solubilization including properties of detergents model lipid systems and detergent-protein interactions; in addition a possible sequence of events when deterGents interact with biological membranes receives attention.

TL;DR: FO and FV are the same type of fluorescence, both emanating from the bulk chlorophyll of Photosystem II, according to simple theory, which predicts that the ratio FV/FM should equal phipo.

TL;DR: Blood platelets contain a lipoxygenase which converts eicosapolyenoic acids with at least two cis double bonds at the 8- and 11-position into l -12-hydroperoxy acids, and the enzyme responsible is found in the supernatant of broken platelets.

TL;DR: Polyethylene glycol quantitatively precipitates plasmid DNA of molecular weight 6-123-10-6 from cleared lysates ofplasmid-carrying bacterial strains, unchanged in length and in transformation efficiency for Escherichia coli K12.

TL;DR: It is concluded that the lipid packing in the outer monolayer of the erythrocyte membrane is comparable with a lateral surface pressure between 31 and 34.8 dynes/cm.

TL;DR: The name cochleate lipid cylinders is suggested for the spiral structures formed by fusion of unilamellar vesicles into large sheets which fold spirally to form cylinders in phosphatidylserine preparations.

TL;DR: A variety of proteins have been studied for their ability to interact and alter the thermotropic properties of phospholipid bilayer membranes as detected by differential scanning calorimeter, and it was concluded that in this case the protein was embedded within the bilayer, associating with a limited number of molecules via non-polar interactions, while the rest of the bilayers was largely unperturbed.

TL;DR: Research interest in the milk fat globule membrane has been strong because of its importance in properties of milk and milk products and the relevance of mammary cell membranes to the breast cancer problem.

TL;DR: The results of these experiments are consistent with the interpretation that (1) the electron dense material inside heavy vesicles may be referable to Ca-2+ binding and/or M55 proteins, and (2) light andheavy vesicle may be derived from the longitudinal sections and terminal cisternae of sarcoplasmic reticulum, respectively.

TL;DR: It is shown that intact chloroplasts are able to fix CO2 in the dark, yielding 3-phosphoglycerate, and demonstrates that CO2 fixation in thedark is not possible, unless the pH in the medium is artificially raised to pH 8.8.

TL;DR: Evidence is presented that the unique properties of the enzyme itself, rather than possible contaminations in the purified preparation, are responsible for the observed haemolytic effect.

TL;DR: A simple photochemical model for the photosynthetic units of Photosystem II based on first-order rate constants for de-excitation of excited chlorophyll molecules is presented in the form of equations which predict the yields of fluorescence.

TL;DR: A good correlation was obtained between fluorescence polarization data on dibucaine-induced changes in membrane fluidity and calorimetric measurements on vesicles of the same type.

TL;DR: Planar bilayer membranes were formed from monolayers of a series of mono-unsaturated monoglycerides and lecithins and showed a negligible voltage dependence, whereas the Cm increased significantly at a voltage of 150 mV in the case of Mueller-Rudin-type monoolein films with n-decane as a solvent.

TL;DR: It is concluded that the regions of the large chain exposed in the presence of K+ are distinct from the regions exposed in presence of Na+ and that two conformations of (Na+, K+)-ATPase can be sensed with trypsin, a (T)K-form and a (t)Na-form.

TL;DR: The major storage protein of the soybean, glycinin, has been prepared in a homogeneous form and examined by a variety of techniques and revealed three different kinds of acidic subunits and probably three kinds of basic ones also.

TL;DR: The Michaelis-Menten kinetics of blood-brain barrier transport of fourteen amino acids was investigated with a tissue-sampling, single-injection technique in the anesthetized rat to determine transport V values and determine brain clearance of tracer concentration of amino acid.

TL;DR: Investigation of the essential structural features of the cold-insoluble globulin of human plasma showed that it is a glycoprotein containing all of the amino acids usually found in proteins, consistent with speculation that the smaller subunit may be a catabolic intermediate arising via release of peptide material containing the COOH-terminus of a parent chain.

TL;DR: It is proposed that the enzyme with iron in the ferric state is the active species in the aerobic and anaerobic reaction of soybean lipoxygenase-1.

TL;DR: It is concluded that the increased permeability at the transition temperature cannot be ascribed to "macroscopic" rupture of the liposomal membrane, and the maximum permeability in the vicinity of the Tc is discussed in terms of probability and size distribution of statistical pore formation at the boundaries of liquid and solid domains.

TL;DR: The collagen produced in response to an injury of human skin is initially stabilized by a crosslink derived from hydroxyallysine, and characteristic of embryonic skin this article, which is typical of young skin collagen.

TL;DR: None of the activities appear to have a requirement for Ca-2-+, and hence would not seem to be involved with active Ca- 2-+ transport in the typical manner, and the concept that the Mg-2+-ATPase and pyrophosphatase activities of matrix vesicles stem from one enzyme, namely, alkaline phosphatase is consistent.

TL;DR: The cyclic peptide phalloidin, one of the toxic components of Amanita phalloides prevented the drop of viscosity of F-actin solutions after the addition of 0.6 M KI and inhibited the ATP splitting of F -actin during sonic vibration.

TL;DR: The non-ionic detergent octyl glucoside solubilizes a substantial amount of Streptococcus faecalis membrane protein without loss of the monitored enzyme activities and the effect of ionic strength indicates that it may be possible to selectively extract groups of membrane proteins by their characteristic solubility at different ionic strengths.