Journal ArticleDOI
Novel Umami Peptide IPIPATKT with Dual Dipeptidyl Peptidase-IV and Angiotensin I-Converting Enzyme Inhibitory Activities.
TLDR
A novel umami peptide, IPIPATKT, showed excellent dual dipeptidyl peptidase-IV (DPP-IV) and angiotensin I-converting enzyme (ACE) inhibitory activities, the IC50 values were 64 and 265 μM, respectively as discussed by the authors.Abstract:
A novel umami peptide, IPIPATKT, showed excellent dual dipeptidyl peptidase-IV (DPP-IV) and angiotensin I-converting enzyme (ACE) inhibitory activities, the IC50 values were 64 and 265 μM, respectively. Molecular docking displayed that IPIPATKT was docked into the S1 and S2 pockets of ACE, and it was close to the active site pocket of DPP-IV. The insulin-resistant-HepG2 (IR-HepG2) cell model and human umbilical vein endothelial cell (HUVEC) model showed that the peptide significantly increased the content of glucose, the activities of hexokinase, pyruvate kinase, and the concentration of nitric oxide (p < 0.01), while it reduced the content of endothelin-1 (ET-1). IPIPATKT exhibited a hypotensive effect (-23.5 ± 2.2 mmHg) and attenuated the increase in glucose levels in vivo, as demonstrated using spontaneous hypertensive rats (SHRs) and C57BL/6N mice. We reported the in vivo activities of the umami peptide with dual hypertensive and hypoglycemic effects for the first time.read more
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Preparation, Characterization, and Cytoprotective Effects on HUVECs of Fourteen Novel Angiotensin-I-Converting Enzyme Inhibitory Peptides From Protein Hydrolysate of Tuna Processing By-Products
TL;DR: Zhang et al. as discussed by the authors used skipjack tuna (Katsuwonus pelamis) processing byproducts to prepare peptides with high angiotensin-I-converting enzyme (ACE) inhibitory (ACEi) activity.
Journal ArticleDOI
Exploration of Dipeptidyl Peptidase-IV (DPP-IV) Inhibitory Peptides from Silkworm Pupae (Bombyx mori) Proteins Based on In Silico and In Vitro Assessments.
Fali Luo,Yu Fu,Liang Ma,Hongjie Dai,Hongxia Wang,Hai Chen,Hank Zhu,Yong-Hua Yu,Yong Hou,Yuhao Zhang +9 more
TL;DR: In silico analysis combined with in vitro assessments can serve as an effective and rapid approach for discovery of DPP-IV peptides from silkworm pupae proteins.
Journal ArticleDOI
Research progress in the screening and evaluation of umami peptides.
TL;DR: In this article , a review summarizes the umami receptors and umami peptides, focusing on the problems restricting the development of the peptide, high-throughput screening, and establishment of evaluation standards.
Journal ArticleDOI
ACE inhibitory activities of two peptides derived from Volutharpa ampullacea perryi hydrolysate and their protective effects on H2O2 induced HUVECs injury.
TL;DR: In this article , the effects of IVTNWDDMEK and VGPAGPRG, two ACE inhibitory peptides purified from Volutharpa ampullacea perryi, on ACE's two domains and on nitric oxide (NO), endothelin-1(ET-1) production in human vascular endothelial cells were explored.
Journal ArticleDOI
Isolation, identification, molecular docking analysis, and cytoprotection of seven novel angiotensin I-converting enzyme inhibitory peptides from miiuy croaker byproducts-swim bladders
TL;DR: In this paper , the authors used the processing byproducts of miiuy croaker to prepare novel angiotensin I-converting enzyme inhibitory (ACEi) peptides, in vitro gastrointestinal (GI) digestion method was screened and employed to prepare swim bladder hydrolysate with the highest ACEi activity.
References
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Journal ArticleDOI
Structural and functional characterization of hemp seed (Cannabis sativa L.) protein-derived antioxidant and antihypertensive peptides
Abraham T. Girgih,Rong He,Rong He,Sunday A. Malomo,Marina Offengenden,Jianping Wu,Rotimi E. Aluko +6 more
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Evaluation of the potential of dietary proteins as precursors of dipeptidyl peptidase (DPP)-IV inhibitors by an in silico approach
TL;DR: It is suggested that dietary proteins could be used for generating DPP-IV inhibitory peptides, and caseins from cow’s milk and collagens from bovine meat and salmon appeared to be the richest potential sources.
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Dipeptidyl Peptidase IV and Its Inhibitors: Therapeutics for Type 2 Diabetes and What Else?
TL;DR: The biological functions and potential substrates of DPPIV enzymes are reviewed and the characteristics of the DPP IV inhibitors are discussed in view of type 2 diabetes and further therapeutic interest.
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Isolation and identification of casein-derived dipeptidyl-peptidase 4 (DPP-4)-inhibitory peptide LPQNIPPL from gouda-type cheese and its effect on plasma glucose in rats
TL;DR: In this paper, a water-soluble extract of a gouda-type cheese showed dipeptidyl-peptide 4 (DPP-4)-inhibitory activity, which should improve glucose tolerance in type 2 diabetes.
Journal ArticleDOI
Identification of a novel ACE-inhibitory peptide from casein and evaluation of the inhibitory mechanisms.
TL;DR: A novel angiotensin I-converting enzyme (ACE)-inhibitory (ACEI) peptide, NMAINPSKENLCSTFCK, derived from the αs2-casein fragment residues 25-41, was screened and identified by UPLC-ESI-Q-TOF-MS/MS from tryptic casein hydrolysate and showed that this peptide acted as a mixed-type inhibitor against ACE.