Patent
Nuclear-encoded transcription system in plastids of higher plants
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TLDR
In this paper, the authors describe DNA constructs and methods for stably transforming the plastids of higher plants. But the constructs described in this paper contain unique promoters that are transcribed by both nuclear encoded PLASTid polymerases and plastid encoded PLastid polymers.Abstract:
The present invention provides novel DNA constructs and methods for stably transforming the plastids of higher plants. The constructs described herein contain unique promoters that are transcribed by both nuclear encoded plastid polymerases and plastid encoded plastid polymerases. Use of the novel constructs of the invention facilitates transformation of a wider range of plant species and enables tissue specific expression of a transforming DNA in plastids of multicellular plants.read more
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References
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Journal ArticleDOI
High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
Zora Svab,Pal Maliga +1 more
TL;DR: The ability to transform routinely plastids of land plants opens the way to manipulate the process of photosynthesis and to incorporate novel genes into the plastid genome of crops.
Journal ArticleDOI
Dynamic regulation of chloroplast transcription
TL;DR: This paper examines the coordinated expression of plastid and nuclear genes for chlorplast development and provides an opportunity to understand how plants sense and alter gene expression in response to light.
Patent
Controlled expression of transgenic constructs in plant plastids
TL;DR: In this paper, a method for controlling the timing or tissue pattern of expression of foreign DNA sequences inserted into the plant plastid genome is described. But this method requires the insertion of the inserted DNA constructs in a tissue and/or developmental specific manner in plants.
Journal ArticleDOI
Chloroplast rps15 and the rpoB/C1/C2 gene cluster are strongly transcribed in ribosome-deficient plastids : evidence for a functioning non-chloroplast-encoded RNA polymerase
TL;DR: The prokaryotic plastid genome of higher plants is transcribed by two RNA polymerases, and the observed differences in transcription between chloroplasts and undifferentiated plastids might reflect different functions of the two enzymes.
Patent
Method for stably transforming plastids of multicellular plants
Pal Maliga,Zora S. Maliga +1 more
TL;DR: In this article, the plastid of a multicellular plant has been stably transformed by a foreign DNA of interest using selectable markers, some of which are non-lethal and some of them are expressible only from the plant genome and not from the nuclear genome.