Journal ArticleDOI
Orosomucoid as one of the serum components contributing to normal capillary permselectivity in rat skeletal muscle
Börje Haraldsson,B. Rippe +1 more
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TLDR
Orosomucoid is one of the components in serum (besides albumin) needed for the maintenance of normal permselectivity of the capillary walls of rat skeletal muscle and is structurally related to other substances exerting this 'serum effect'.Abstract:
The effects of human serum orosomucoid (normal serum concentration 0.7-1.0 g l-1) on capillary permeability were investigated in 12 isolated maximally vasodilated rat hindquarters perfused with bovine serum albumin (50 g l-1) in modified Tyrode. Measurements were made of capillary filtration coefficient (CFC), permeability surface area product (PS) for vitamin B12 and isogravimetric clearance of radiolabelled albumin (Cl alb). The results were compared with those obtained using perfusates without addition of orosomucoid ('albumin group') or perfusates containing horse serum ('serum group'). Clearance of albumin was almost four-fold higher in the albumin than in the serum group, 0.0895 +/- 0.0066 (n = 12) and 0.0252 +/- 0.016 ml min-1 per 100 g (n = 18), respectively, while intermediate Cl alb values were obtained with human orosomucoid in the perfusate (greater than 0.1 g l-1), 0.0436 +/- 0.0034 ml min-1 per 100 g) (n = 8). These changes in Cl alb were not accompanied by any differences in CFC or PS. We conclude that orosomucoid is one of the components in serum (besides albumin) needed for the maintenance of normal permselectivity of the capillary walls of rat skeletal muscle. Alternatively, human orosomucoid is structurally related to other substances exerting this 'serum effect'.read more
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References
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Journal ArticleDOI
The Permeability of Capillaries in Various Organs as Determined by Use of the ‘Indicator Diffusion’ Method
TL;DR: It is shown that the permeability of a capillary area can be expressed by three parameters: the initial extraction of test substances added in a single injection to the blood flowing to an organ, the blood flow and the surface area of the capillaries.
Journal ArticleDOI
Glycosaminoglycans and their binding to biological macromolecules.
Ulf Lindahl,Magnus Höök +1 more
TL;DR: STRUCTURE and BIOSYNTHESIS of GL YCOSAMINOGL YCANS: Foundations and biosynthesis of GL ycans.
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Transport of potassium-42 from blood to tissue in isolated mammalian skeletal muscles.
TL;DR: A method is described for studying transcapillary diffusion of K42 in isolated perfused muscles of dogs and its effects on blood flow and arteriovenous K42 differences are measured.
Journal ArticleDOI
Effective osmotic pressure of the plasma proteins and other quantities associated with the capillary circulation in the hindlimbs of cats and dogs
TL;DR: This behavior represents a special case of the Starling theory in which tissue pressure opposing filtration is negligible and the capillary membranes are almost completely impermeable to the plasma proteins; it implies that the protein concentration in tissue fluid immediately outside the capillaries is too low to play a significant role in determining the osmotic balance.
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