Oxygen Evolved by Isolated Chloroplasts

TLDR: The hæmoglobin method, originally used by Hoppe-Seyler to demonstrate oxygen from green plants, has been applied to study the oxygen evolution of isolated chloroplasts exposed to light.

Abstract: THE high affinity for oxygen possessed by muscle haemoglobin suggested its use as a very sensitive spectroscopic method for detecting and measuring small quantities of oxygen1. This method has now been applied to study the oxygen evolution of isolated chloroplasts exposed to light. While being much less sensitive than the bacterial methods which have been successfully applied in the past, the haemoglobin method (originally used by Hoppe-Seyler to demonstrate oxygen from green plants) has the advantage of giving the measure of oxygen. A solution of haemoglobin containing 0.45 × 104 gm. atoms of iron per litre, is equivalent to 1 c.mm. of oxygen per c.c.; the degree of saturation can be determined spectroscopically with an accuracy of 5 per cent.