Journal ArticleDOI
Perspectives report: Melting of DNA. Its study and application as a research method
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This article is published in Biopolymers.The article was published on 1970-11-01. It has received 186 citations till now.read more
Citations
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Journal ArticleDOI
Thermal denaturation of DNA molecules: A comparison of theory with experiment
TL;DR: In this article, experimental and theoretical results on the helix-coil transition of DNA are reviewed and the theoretical model of the transition is described, and the influence of heterogeneous base pair stacking and strand dissociation on the predicted melting transition is examined.
Journal ArticleDOI
Energetics of drug-dna interactions
TL;DR: A perspective is offered on the interpretation of these thermodynamics parameters, and in particular how they might be correlated with known structural features on the drug-DNA binding process.
Book ChapterDOI
Reactions of nucleic acids and nucleoproteins with formaldehyde.
TL;DR: The chapter emphasizes on the evidence for the formation of various structures and the molecular mechanisms of biological and other effects of formaldehyde, one of the most promising mutagenic agents affecting multicellular organisms.
Journal ArticleDOI
Theoretical calculations of the helix-coil transition of DNA in the presence of large, cooperatively binding ligands.
TL;DR: The denaturation of poly[d(A‐T)] in the presence of the drug, netropsin, is used to verify some features of the theory and to illustrate how the theory can be used to obtain numerical estimates of the ligand binding parameters from the experimental melting curves.
Journal ArticleDOI
The B̄ to Ā transition of DNA in solution
TL;DR: On the basis of data obtained in the presence of the antibiotic netropsin, which stabilizes the B form, the co-operativity length, ν0 = 20 base pairs, and the free energy difference of the Ā and B states in water are estimated.
References
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Journal ArticleDOI
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
J. Marmur,Paul Doty +1 more
TL;DR: The previously discovered linear relation between the base composition of DNA, expressed in percentage of guanine plus cytosine bases, and the denaturation temperature, T m, has been further investigated and it appears that the measurement of the T m is a satisfactory means of determining base composition in DNA.
Journal ArticleDOI
Intramolecular Reaction in Polycondensations. I. The Theory of Linear Systems
TL;DR: For three types of linear polycondensing systems, equilibrium molecular size distributions, including rings as allowable species, are derived in this article, and average molecular weights and amounts of ring and chain fractions are calculated therefrom.
Journal ArticleDOI
Pyrimidine dimers in ultraviolet-irradiated DNA's.
TL;DR: DNA's of various compositions, labeled with radioactive cytosine or thymine, were irradiated with monochromatic ultraviolet light and analyzed by photochemical and chromatographic techniques for photoproducts to identify pyrimidine dimers, which interfere with nuclease action and inhibit DNA synthesis in vitro.
Journal ArticleDOI
Theory of the melting transition of synthetic polynucleotides: Evaluation of the stacking free energy
Donald M. Crothers,Bruno H. Zimm +1 more
TL;DR: Applying the theory to the results of Inman & Baldwin (1964) on dAT† and mixtures of dC and dI, it is estimated that the stacking free energy must be a major part of the free energy stabilizing the helix.
Journal ArticleDOI
Early and late helix-coil transitions in closed circular DNA. The number of superhelical turns in polyoma DNA.
TL;DR: It is calculated that the closed circular molecule contains −15 ± 1 superhelical turns in buoyant cesium chloride at neutral pH, and it is estimated that the melting temperature of polyoma I in standard saline citrate should be 107 °C.
Related Papers (5)
Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.
J. Marmur,Paul Doty +1 more
Theory of the melting transition of synthetic polynucleotides: Evaluation of the stacking free energy
Donald M. Crothers,Bruno H. Zimm +1 more