Showing papers in "Journal of Molecular Biology in 1974"

TL;DR: The results indicate that the binding of any non-interacting ligand covering more than one lattice residue results in non- linear (convex downward) Scatchard plots, and the introduction of positive ligand-ligand co-operativity antagonizes this non-linearity, and eventually leads to plots of the opposite curvature.

TL;DR: The thermal properties of five globular proteins with known spatial structure, ribonuclease, lysozyme, chymotrypsin, cytochrome c and myoglobin, are investigated by scanning microcalorimetry and it is shown that heat-denaturation of these proteins can be described to a first approximation by the two-state transition model.

TL;DR: The salivary glands and other tissues from Drosophila melanogaster were dissected at various times throughout the prepupal period, as well as after heat shocks and ecdysterone treatments, and the proteins labelled by incubating the isolated tissues with [35S]methionine were separated by electrophoresis on sodium dodecyl sulphate-polyacrylamide gel.

TL;DR: It is suggested that simple geometrical packing considerations may provide useful criteria in guiding and evaluating trial structures in theoretical studies of protein folding, especially the association of distant parts of a peptide chain.

TL;DR: Turbidity measurements have been used to study the in vitro assembly and disassembly of porcine neurotubules and GTP, ATP and ADP, but not GDP, are effective in promoting tubule assembly, which is essentially fully reversible in the presence of excess GTP.

TL;DR: The hypochromicity, as a function of temperature for 19 oligoribonucleotides capable of forming perfectly base-paired double helices, is used to extract thermodynamic parameters of helix formation.

TL;DR: A double helix model is proposed that accounts for the sign and magnitude of the optical rotation shift that accompanies the sol-gel transitions and is sterically accessible to each of the various substituted forms.

TL;DR: Determination of cholesterol revealed that its relative amount in a lymphoma cell is about half of that of a normal lymphocyte, a difference that may account for the above difference in fluidity.

TL;DR: An objective assessment of the upper limit of the error in the atomic coordinates of the final model is possible by comparing the inhibitor component in the model of the complex with the refined structure of the free inhibitor (Deisenhofer & Steigemann, 1974).

TL;DR: The hypothesis is formulated that the large plasmid present in crown-gall inducing bacteria could be the “tumor-inducing principle”.

TL;DR: Algorithms are suggested for identifying α-helical and β-structural regions in native globular proteins with accuracy of ~80 and ~85% respectively for 25 proteins, the three-dimensional structures of which have been determined by X-ray diffraction crystallography.

TL;DR: From the order of the fragments formed by EcoRI and Hpa I on the adenovirus 2 map, it is concluded that these cell lines contain only the segment of viral DNA that stretches from the left-hand end to a point about 14% along the viral genome, which must be coded by any viral function expressed in transformed cells.

TL;DR: X-ray diffraction analyses of fibers of polydeoxyadenylic acid · polydeoxythymidylic acid show that this molecule exists as a 10-fold double-helix with axial rise per nucleotide h = 3.24 to 3.29 A.

TL;DR: The sequence relations between some bacterial F-prime factors in Escherichia coli have been determined by observing the pattern of duplex and single-stranded regions in heteroduplexes consisting of one strand from one episome and the complementary strand from another.

TL;DR: The conclusion of the theoretical analysis and the experimental results is that the binding of an ethidium molecule to a double-stranded DNA unwinds the DNA helix by an angle φe = 26 °, approximately a factor of two larger than the value 12 °, which has been in use in the past decade.

TL;DR: Experiments involving the shift of mutant cells from the restrictive to the permissive temperature in the presence of cycloheximide demonstrated that the protein synthesis requirement for yeast DNA replication can be completed before the cdc 7-mediated step.

TL;DR: An a priori theory is developed which permits the identification, in good agreement with experiment, of α-helical and β-structural regions in globular proteins from their primary structure.

TL;DR: Stroud et al. as mentioned in this paper reported the progress of analysis from the low-resolution (5 A) model, which shows the general molecular architecture, through high-resolution density maps, from which a model of the enzyme was built.

TL;DR: It is concluded that if duck DNA does contain poly(dA) sequences corresponding to mRNA-associated poly(rA) sequence, there are fewer than 8000 of these per haploid genome.

TL;DR: On the basis of data obtained in the presence of the antibiotic netropsin, which stabilizes the B form, the co-operativity length, ν0 = 20 base pairs, and the free energy difference of the Ā and B states in water are estimated.

TL;DR: Low-resolution X-ray analysis of the binding of thyroxine and tri-iodothyronine to prealbumin shows that the hormones are bound in two symmetry-related binding sites located deeply in the central slot.

TL;DR: The results show that the principal path for the reversible thermal unfolding of tRNA 1 fMet under these solution conditions is first, transient opening of the dihydrouridine helix, followed by simultaneous melting of the diazepam helix and a “tertiary” interaction, which does not correspond to a cloverleaf helix.

TL;DR: It is proposed that this cell-specificity reflects a situation in which HnRNA molecules that differ in nucleotide sequence are complexed with different sets of proteins, so that the resulting HnRNP particles are biochemically distinct at each genetic locus.

TL;DR: Hypertonicity effected by elevation of NaCl concentration in the growth medium results in a rapid cessation of protein synthesis in HeLa cells accompanied by a complete breakdown of polyribosomes, which allowed us to determine the gene sequence of poliovirus RNA in a novel way.

TL;DR: The microtubules of flagella have an intrinsic polarity reflected in their side-arm attachments and in their directionality of growth, as well as a limited extent of proximal addition and growth on the B-tubule.

TL;DR: The crystal structure of lobster d -glyceraldehyde-3-phosphate dehydrogenase has been analyzed and it is shown that the amino acids in the active center region and involved in subunit-subunit contacts are more highly conserved than other residues.

TL;DR: The quantity of poly(A)-containing RNA is measured in Xenopus laevis oocytes as a function of developmental stage and remains relatively constant from early vitellogenesis until ovulation.

TL;DR: A series of isonuclear cytoplasmic petite (ϱ−) mutants presenting various patterns of large deletions and retentions in the C321R — E514R region was used to study the functional organization of mitochondrial DNA.

TL;DR: The energy refinement of hen egg-white lysozyme co-ordinates is described, a process in which atoms are moved to reduce the molecular potential energy and give a stereochemically acceptable set of atomic co-ords.

TL;DR: The presence of such long, well-matched palindromes suggests that the linear double helix may sometimes adopt an alternative configuration, the cruciform, in which mismatches that may occur are eliminated by excision and repair.