Journal ArticleDOI
Pleiotropic mutants affecting the control of nitrogen metabolism in Aspergillus nidulans
TLDR
Mutants of Aspergillus nidulans with lesions in gene amdT are pleiotropically affected in their ability to utilize a wide variety of nitrogen sources in the presence of glucose, with properties consistent with it being derepressed for glucose repression of the utilization of most (but not all) nitrogen sources.Abstract:
Mutants of Aspergillus nidulans with lesions in gene amdT are pleiotropically affected in their ability to utilize a wide variety of nitrogen sources in the presence of glucose. Ability to utilize a number of these compounds as sole sources of carbon and nitrogen is not altered. One of these mutants, amdT102, has properties consistent with it being derepressed for glucose repression of the utilization of most (but not all) nitrogen sources. The amdT102 mutant can grow strongly on histidine, lysine and cystine as sole nitrogen sources while the wild type strain grows extremely poorly on these amino acids. Similar but less extreme effects apply to many other nitrogen sources. The amdT19 mutant is unable to utilize most nitrogen sources in the presence of glucose, suggesting that it is subject to greatly increased repression of nitrogen source utilization. The amdT mutants are not affected in their ability to use many compounds as sole carbon sources. Carbon sources other than glucose also affect utilization of nitrogen sources in the amdT mutants.read more
Citations
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Journal ArticleDOI
The regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans. Mutations affecting specificity of gene activation alter a loop residue of a putative zinc finger.
B Kudla,Mark X. Caddick,Tim Langdon,Nilce Maria Martinez-Rossi,C F Bennett,S Sibley,R W Davies,Herbert N. Arst +7 more
TL;DR: Results show that the regulatory gene areA mediating nitrogen metabolite repression in Aspergillus nidulans reverts to a partly reciprocal phenotype by replacing the mutant valine by methionine, and also identifies sequence changes associated with specificity mutations.
Journal ArticleDOI
Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations.
TL;DR: The regulatory mutations in the controlling region of the amdS gene were found to be due to small-scale alterations in the DNA rather than to large-scale rearrangements resulting in gene fusions.
Journal ArticleDOI
Pleiotropic mutants of Aspergillus nidulans altered in carbon metabolism.
Michael J. Hynes,Joan M. Kelly +1 more
TL;DR: The results indicate that thecreB andcreC genes may have a regulatory role in the control of carbon catabolism.
Journal ArticleDOI
Mutational Analysis of AREA, a Transcriptional Activator Mediating Nitrogen Metabolite Repression in Aspergillus nidulans and a Member of the “Streetwise” GATA Family of Transcription Factors
TL;DR: Tolerance to sequence change (with retention of function) is surprisingly great, and mutational changes in a transcription factor can have widely differing, even opposing, effects on expression of different structural genes so that monitoring expression of one or even several structural genes can be insufficient and possibly misleading.
Journal ArticleDOI
Multiple copies of the amdS gene of Aspergillus nidulans cause titration of trans-acting regulatory proteins
Joan M. Kelly,Michael J. Hynes +1 more
TL;DR: It has been established that a plasmid containing the amdS gene of Aspergillus nidulans may be used to transform amdS+ strains by selecting for increased utilization of acetamide as sole nitrogen source.
References
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Journal ArticleDOI
The induction and repression of nitrate reductase in the fungus Aspergillus nidulans.
TL;DR: The result of experiments indicate that the synthesis of nitrate reductase is induced by nitrate and repressed by ammonium, and no effect in vitro of ammonium could be found.
Journal ArticleDOI
Mechanism of activation of catabolite-sensitive genes: a positive control system.
TL;DR: It is concluded that cyclic AMP and a protein factor called the catabolite gene activator protein are part of a positive control system for activating catabolites-sensitive genes.
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Cyclic AMP Receptor Protein of E. coli: Its Role in the Synthesis of Inducible Enzymes
TL;DR: A cyclic AMP binding protein has been purified over 100-fold from E. coli extracts as mentioned in this paper, and this binding protein appears to be required for cyclicAMP action, they suggest it be called the cyclic amp receptor protein (CR protein).
Cyclic AMP Receptor Protein ofE.coli:ItsRoleinthe Synthesis ofInducible Enzymes
TL;DR: The addition of purified, wild-type binding protein to these extracts restores enzyme synthesis toward normal and is suggested to be called the cyclic AMP receptor protein (CR protein), because this binding protein appears to be required for cyclicAMP action.
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Resistance to catabolite repression of histidase and proline oxidase during nitrogen-limited growth of Klebsiella aerogenes.
TL;DR: It is concluded that in K. aerogenes certain catabolitesensitive enzymes which degrade nitrogenous compounds are synthesized rapidly in the presence of glucose if the supply of nitrogen is limited.