Polarity of flagellar growth in salmonella.
TLDR
The authors speculate that E. coli cells die off in the course of starvation not because some unique structure is destroyed, but owing to the fact that the activity of enzymes and ribosomes gradually declines, and the synthetic activity of the cell drops down abruptly and irreversibly.Abstract:
The work is concerned with studying the breakdown of proteins and RNA when a polyauxotrophic Escherichia coli strain is incubated in a salt solution without amino acids, phosphorus, nitrogen and glucose at 43 degrees C as well as the ability of starving bacterial cells to recommence protein and RNA synthesis (also in the course of phage T4 infection) and to reproduce bacteriophages T4, lambda and MS2. Within the first two hours of the incubation, 12% of proteins and 40% of RNA break down to acid-soluble fragments. Then protein degradation stops while RNA decomposition goes on, but at a lower rate. Within 4-6 h of starvation, the rate of protein and RNA synthesis drops down 4-5 times and the survival rate equals 40-60% when the cells are transferred onto a complete medium. The quantitative characteristics of phages T4, lambda and MS2 reproduction fall down in prestarved cells. The authors speculate that E. coli cells die off in the course of starvation not because some unique structure is destroyed, but owing to the fact that the activity of enzymes and ribosomes gradually declines. As a result, the synthetic activity of the cell drops down abruptly and irreversibly because the enzymes are inactivated and RNA breaks down, which eventually causes cell death.read more
Citations
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Journal ArticleDOI
The Rotary Motor of Bacterial Flagella
TL;DR: Flagellated bacteria, such as Escherichia coli, swim by rotating thin helical filaments, each driven at its base by a reversible rotary motor, powered by an ion flux.
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Coordinating assembly of a bacterial macromolecular machine
TL;DR: This mechanism that controls levels of the bacterial flagellar regulatory protein FlgM is discussed, together with others that also coordinate gene regulation and flageLLar assembly in Gram-negative bacteria.
Journal ArticleDOI
Process of Protein Transport by the Type III Secretion System
TL;DR: The type III secretion system of gram-negative bacteria is responsible for delivering bacterial proteins, termed effectors, from the bacterial cytosol directly into the interior of host cells and shows functional complementarity for secretion and translocation.
Journal ArticleDOI
Regulation of flagellar motility during biofilm formation
TL;DR: The regulation of motility during biofilm formation in Bacillus, Pseudomonas, Vibrio, and Escherichia is reviewed, and it is concluded that the motility-to-biofilm transition, if necessary, likely involves two steps.
References
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Journal ArticleDOI
Staining, shape and arrangement of bacterial flagella.
TL;DR: The flagella staining technique to be described is a modification and perfection of that originally published by the author in 1930 and appears to be a characteristic of sufficient stability and distinctiveness to justify the classification of flagellated bacteria into several groups.
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Reconstitution of bacterial flagella in vitro
TL;DR: The rate of growth of flagellar filaments in vitro is determined under various salt conditions and found to be comparable to that observed in vivo.
Journal ArticleDOI
Salmonella flagella: in vitro reconstruction and over-all shapes of flagellar filaments.
TL;DR: It is concluded that the normal type filament possesses an intrinsic ability to transform between the normal and curly types, depending on external conditions, and that this transformation is akin to dimorphic transition in crystals.
Journal ArticleDOI
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TL;DR: It was concluded that during reconstitution flagellar filaments grow in a unidirectional manner.
Journal ArticleDOI
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TL;DR: The flagellation and motility of Salmonella typhimurium were unaffected by proteolytic enzymes; but when washed stationary-phase bacteria were briefly exposed to acid the proportion of motile, and of flageLLated, bacteria was decreased from more than 50% to less than 1%, with little or no decrease in viable count.
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