Journal ArticleDOI
Polymorphisms of coding region of BMPR - IB gene and their relationship with litter size in sheep
M. X. Chu,Lihua Jia,Yingjie Zhang,Mei Jin,Hong-Quan Chen,L. Fang,R. Di,G. L. Cao,T. Feng,Qianqian Tang,Yuehui Ma,Kui Li +11 more
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TLDR
It is preliminarily demonstrated that BMPR-IB is a major gene affecting the hyperprolificacy in Small Tail Han and Hu sheep, and could be used as a molecular genetic marker for early auxiliary selection for hyperprolate selection in sheep.Abstract:
The bone morphogenetic protein receptor IB (BMPR-IB) was studied as a candidate gene for the prolificacy of sheep. Nine pairs of primers (P1–P9) were designed to detect single nucleotide polymorphisms (SNPs) of exons 1–4 and 6–10 of the BMPR-IB gene in both high (Small Tail Han and Hu sheep) and low prolificacy breeds (Texel and Chinese Merino sheep) by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP). Only the products amplified by primers P2, P5, P6, P7, P8 and P9 displayed polymorphisms. The present study identified 22 SNPs in partial coding regions of ovine BMPR-IB, in which 20 SNPs were reported for the first time. In total of the 22 mutations, 18 DNA variations were originated from the Hu breed, three were found in the Small Tail Han breed (two of them were found in other sheep breeds), three in the Chinese Merino breed, and none in the Texel breed. These results preliminarily demonstrated that BMPR-IB is a major gene affecting the hyperprolificacy in Small Tail Han and Hu sheep, and could be used as a molecular genetic marker for early auxiliary selection for hyperprolificacy in sheep.read more
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Genome-Wide Association Analyses Highlight the Potential for Different Genetic Mechanisms for Litter Size Among Sheep Breeds.
Song-Song Xu,Lei Gao,Xing-Long Xie,Yan-Ling Ren,Zhi-Qiang Shen,Feng Wang,Min Shen,Emma Eyϸórsdóttir,Jón Hallsteinn Hallsson,Tatyana Kiseleva,Juha Kantanen,Meng-Hua Li +11 more
TL;DR: The results provide new insights into the genetic mechanisms underlying the prolificacy trait in sheep and other mammals, suggesting targets for selection where the aim is to increase prolificacy in breeding projects.
Journal ArticleDOI
RFamide Peptides: Structure, Function, Mechanisms and Pharmaceutical Potential
TL;DR: The review summarizes the reported peptide analogs and recently developed small molecule ligands (agonists and antagonists) to highlight the current understanding of the pharmacophoric elements, required for affinity and activity at the receptor family.
Journal ArticleDOI
Genome-wide transcriptome analysis of mRNAs and microRNAs in Dorset and Small Tail Han sheep to explore the regulation of fecundity.
TL;DR: This genome-wide analysis of mRNAs and miRNAs in sheep will aid in the ability to identify fecundity regulators between different sheep species and is the first analysis of intravariety and intervariety in any species in this area.
Journal ArticleDOI
Genome-wide analysis of microRNAs identifies the lipid metabolism pathway to be a defining factor in adipose tissue from different sheep.
TL;DR: Gene ontology and pathway analyses of the predicted target genes that negatively associated with the differentially expressed miRNAs revealed that there was less active lipid metabolism in the adipose tissue of Small Tail Han sheep.
Journal ArticleDOI
Ovarian proteomic study reveals the possible molecular mechanism for hyperprolificacy of Small Tail Han sheep.
TL;DR: An iTRAQ-based quantitative proteomics analysis was constructed to compare the ovarian proteomes of FecB+FecB- genotype Small Tail Han sheep ewes (Han ++), FecBBFecBB Han ewers (Han BB) and Dorset eWes (Dorset), providing a prospective understanding of the molecular mechanism for high prolificacy.
References
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Journal ArticleDOI
Protein kinases 6. The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification.
Steven K. Hanks,Tony Hunter +1 more
TL;DR: The eukaryotic protein kinases make up a large superfamily of homologous proteins, and a classification scheme can be founded on a kinase domain phylogeny, which reveals families of enzymes that have related substrate specificities and modes of regulation.
Journal ArticleDOI
Highly Prolific Booroola Sheep Have a Mutation in the Intracellular Kinase Domain of Bone Morphogenetic Protein IB Receptor (ALK-6) That Is Expressed in Both Oocytes and Granulosa Cells
T. Wilson,Xi-Yang Wu,Jennifer L. Juengel,Ian K. Ross,J. M. Lumsden,E A Lord,Ken G. Dodds,Grant A. Walling,John C. McEwan,Anne R. O'Connell,K. P. McNatty,Grant W. Montgomery +11 more
TL;DR: The mutation in BMPR-IB found in Booroola sheep is the second reported defect in a gene from the TGF-β pathway affecting fertility in sheep following the recent discovery of mutations in the growth factor, GDF9b/BMP15.
Journal ArticleDOI
Mutation in bone morphogenetic protein receptor-IB is associated with increased ovulation rate in Booroola Mérino ewes
Philippe Mulsant,Frédéric Lecerf,Stéphane Fabre,Laurent Schibler,Philippe Monget,Isabelle Lanneluc,Claudine Pisselet,Juliette Riquet,Danielle Monniaux,Isabelle Callebaut,Edmond P. Cribiu,J. Thimonier,J. Teyssier,Loys Bodin,Yves Cognié,Nour Chitour,Jean-Michel Elsen +16 more
TL;DR: It is suggested that in FecBB/FecBB ewes, BMPR-IB would be inactivated partially, leading to an advanced differentiation of granulosa cells and an advanced maturation of ovulatory follicles.
Journal ArticleDOI
The Booroola (FecB) phenotype is associated with a mutation in the bone morphogenetic receptor type 1 B (BMPR1B) gene.
TL;DR: A mutation in the subdomain 3 of the kinase domain could result in an alteration in the expression and/or phosphorylation of SMADs, resulting in the phenotype characteristic of the Booroola animals which is the 'precocious' development of a large number of small antral follicles resulting in increased ovulation rate.
Journal ArticleDOI
Segregation of a major gene influencing fecundity in progeny of Booroola sheep
TL;DR: The results support the hypothesis that the exceptional fecundity of the Booroola arises because, at one of the relevant loci, the majority of Booroolas carry a gene(s) with a major effect on ovulation rate.