Porphyrin binding by the surface array virulence protein of Aeromonas salmonicida.
TLDR
Congo red binding was structurally specific; it was not influenced by a wide variety of compounds including amino acids and nucleotides and only weakly inhibited by structurally similar dyes, however, protoporphyrin IX and hemin were strong competitive inhibitors of Congo red binding.Abstract:
Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells by a kinetically distinct mechanism (Kd, 0.25 microM) which was absent in A- isogenic strains. Purified A-layer protein ("A protein") protein A also bound Congo red with similar affinity (Kd, 0.40 microM). Congo red binding was structurally specific; it was not influenced by a wide variety of compounds including amino acids and nucleotides and only weakly inhibited by structurally similar dyes. However, protoporphyrin IX and hemin were strong competitive inhibitors of Congo red binding. Protoporphyrin and hemin were bound only by A+ strains (KdS of 0.41 and 0.63 microM, respectively). Furthermore, binding of these porphyrins was strongly inhibited by Congo red but weakly inhibited by hematoporphyrin. Purified A protein also bound protoporphyrin IX and hemin with affinities similar to those of A+ cells (KdS of 0.94 and 0.41 microM, respectively.read more
Citations
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References
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Journal ArticleDOI
Loss of virulence during culture of Aeromonas salmonicida at high temperature.
Edward E. Ishiguro,William W. Kay,T Ainsworth,J.B. Chamberlain,R A Austen,J T Buckley,Trevor J. Trust +6 more
TL;DR: It is proposed that the A-layer contributes significantly to the physical properties of the A. salmonicida cell envelope and that these physical properties change upon loss of theA-layer to permit growth at a higher-than-usual temperature.
Journal ArticleDOI
Role of surface components in serum resistance of virulent Aeromonas salmonicida
TL;DR: The ability of virulent strains of Aeromonas salmonicida to resist the bactericidal activity of serum was quantitated and in immune trout serum, the protection was conferred by A-protein.
Journal ArticleDOI
Congo Red-Agar Plating Medium for Detecting Pigmentation in Pasteurella pestis
TL;DR: A new differential medium, containing Congo red dye and common, commercially available laboratory media, is devised, possibly indicating a common binding site for hematin and Congo red, should be useful in efforts to determine the chemical nature of a bacterial component associated with high virulence in P. pestis.
Journal ArticleDOI
Loss of pigmentation in Shigella flexneri 2a is correlated with loss of virulence and virulence-associated plasmid.
TL;DR: The results of this study support the belief that although pigmentation is usually associated with virulence, genetic determinants unrelated to virulence can also affect the ability of the cell to bind Congo red, and theAbility of S. flexneri 2a to binds Congo red does not necessarily imply the ability to invade epithelial cells.
Journal ArticleDOI
Purification and disposition of a surface protein associated with virulence of Aeromonas salmonicida.
TL;DR: A variety of radiolabeling studies showed that this protein was surface localized and that it provided an effective barrier against iodination of other outer membrane proteins with either lactoperoxidase or diazoiodosulfanilic acid.