Journal ArticleDOI
Preservation of intercellular substances by the cationic dye alcian blue in preparative procedures for electron microscopy
O. Behnke,T. Zelander +1 more
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Alcian blue appears to bind nonspecifically to anionic sites in cell coat mucosubstances, with the formation of a rather insoluble, osmiophilic dye-mucosUBstance complex.About:
This article is published in Journal of Ultrastructure Research.The article was published on 1970-06-01. It has received 287 citations till now. The article focuses on the topics: Ruthenium red.read more
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Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action
TL;DR: The results indicate that ruthenium red, as a hexavalent cation, precipitates a large variety of polyanions by ionic interaction, and that its classical reaction with pectin is typical rather than specific.
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Ruthenium red and violet. II. Fine structural localization in animal tissues.
TL;DR: Ruthenium red stains intracellular lipid droplets revealing lamellae, and stains myelin forms grown from crude egg lecithin but cannot penetrate deeply, and is localized in extracellular materials which have an important mechanical function.
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Anionic sites in the glomerular basement membrane. In vivo and in vitro localization to the laminae rarae by cationic probes.
Yashpal S. Kanwar,M G Farquhar +1 more
TL;DR: The experiments demonstrate the existence of a quasi- regular, lattice-like network of anionic sites in the LRI and LRE and the mesangial matrix, demonstrable in vivo (by CF binding), in fixed kidneys (by RR staining), and in isolated GBM's ( by CF binding).
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An Electron-microscopic Demonstration of an Acidic Polysaccharide Involved in the Adhesion of a Marine Bacterium to Solid Surfaces
Madilyn Fletcher,G. D. Floodgate +1 more
TL;DR: Staining a marine bacterium with Ruthenium Red and Alcian Blue demonstrated an extracellular, compact acidic polysaccharide layer, which was involved in bacterial adhesion to surfaces, and appeared to assist adhesion when they were forced into contact with a suitable surface.
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Factors governing adherence of Candida species to plastic surfaces
TL;DR: Biophysical characterization of the ability of Candida albicans and Candida spp.
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Differential staining of acid glycosaminoglycans (mucopolysaccharides) by Alcian blue in salt solutions
J. E. Scott,J. Dorling +1 more
TL;DR: Model experiments with pure polyanions, or artifically carboxylated, phosphorylated and sulphated liver sections, showed that binding of dye to carboxyate or phosphate groups ceased at low electrolyte concentrations, whereas dye continued to be held by sulphate ester groups at concentrations five to ten times as high.
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Electron microscope observations on the carbohydrate-rich cell coat present at the surface of cells in the rat.
A. Rambourg,C. P. Leblond +1 more
TL;DR: Findings indicate that the stained layer is a "cell coat" located outside the plasma membrane, since the cell coat is also stained by colloidal thorium, a technique for detection of acidic carbohydrates, this structure presumably contains not only glycoprotein but also acidic residues.
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The enteric surface coat on cat intestinal microvilli.
TL;DR: The stability, consistent presence, and intimate association of the mucopolysaccharide coat suggest that it may be an integral part of the plasmalemma rather than an "extraneous coat."
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Histochemical Demonstration of Acid Polysaccharides in Animal Tissues
TL;DR: The objective is to establish an experimental procedure and show direct AFM measurements that unequivocally can be assigned as the carrier and removal of polysaccharides from the animal body.