Prevalence and species diversity of nontuberculous mycobacteria in drinking water supply system of Bahía Blanca City, Argentina.
TLDR
It should be taken into account that sequencing of the 16S rRNA gene and biochemical tests are useful for the identification of several species, but it is necessary to incorporate other genes (hsp 65 and rpo B) to obtain accurate identification.Abstract:
Background: There is evidence that tap water is the vehicle through which nontuberculous mycobacteria (NTM) infect or colonize the human body. The objective of this study was to determine the presence and diversity of NTM in the water distribution system of Bahia Blanca city, Argentina (sites S2/S3) and in the dike that supplies water to it (S1). Methods: Culture-dependent method, biochemical tests, and molecular method (16S rRNA sequencing gene) were combined to detect and identify NTM. Results: NTM were isolated in 51.6% (64/124) of all the samples analyzed. Mycobacterium gordonae was the most frequently isolated organism (15/64) in all samples analyzed, followed by Mycobacterium peregrinum and Mycobacterium frederiksbergense. Significant differences were found in the residual chlorine values between sampling S2 and S3. In both sites, maximum counts were recorded but they did not correlate with low chlorine values. A concentration higher than 500 colony-forming unit/L of NTM was never found, which can be attributed to the negative effect caused by decontamination methods being a point to consider for the recovery of NTM. In 46.9% (30/64) of samples, both methods coincided in the identification, and the obtained sequences presented ≥99% identity. Identification at the species level was achieved in 50% (32/64) of the isolates. Nearly 17.2% (11/64) of the isolates showed a similarityread more
Citations
More filters
Journal ArticleDOI
Consensus management recommendations for less common non-tuberculous mycobacterial pulmonary diseases.
Christoph Lange,Erik C. Böttger,Emmanuelle Cambau,David E. Griffith,Lorenzo Guglielmetti,Jakko van Ingen,Shandra L Knight,Theodore K. Marras,Kenneth N. Olivier,Miguel Santin,Jason E. Stout,Enrico Tortoli,Dirk Wagner,Kevin L. Winthrop,Charles L. Daley +14 more
TL;DR: In this article , the authors provided evidence-based recommendations for the treatment of less common non-tuberculous mycobacterial (NTM) species in adult patients without cystic fibrosis or HIV infection.
Journal ArticleDOI
Recovery of Mycobacteria from Heavily Contaminated Environmental Matrices
TL;DR: In this article, a decontamination method using a solution containing 4.0% NaOH and 0.5% tetradecyltrimethylammonium bromide (TDAB) represents a relatively simple and universal procedure for processing heavily microbially contaminated matrices together with increase of mycobacteria yield and elimination of gross contamination.
Journal ArticleDOI
Identification of Nontuberculous Mycobacteria in Drinking Water in Cali, Colombia.
Andrés F. Dávalos,Pamela K. Garcia,Carolina Montoya-Pachongo,Andrea Rengifo,Daniela Guerrero,Lorena Díaz-Ordoñez,Gustavo Diaz,Beatriz E. Ferro +7 more
TL;DR: In this paper, the authors identify and characterize NTM present in tap water samples from Cali, Colombia, including the city's three main water treatment plants (WTPs) including filter-trapped material and eluates (0.45 μm) from swab washes were plated in 7H11 agar plates.
Journal ArticleDOI
Effect of COVID-19 pandemic on incidence of mycobacterial diseases among suspected tuberculosis pulmonary patients in Tehran, Iran
Jafar Aghajani,Poopak Farnia,Parissa Farnia,Jalaledin Ghanavi,Shima Saif,Majid Marjani,Payam Tabarsi,Afshin Moniri,Zahra Abtahian,Sven Hoffner,Ali Akbar Velayati +10 more
TL;DR: In this paper , the authors compared the suspected tuberculosis (TB) pulmonary patients before and during the COVID-19 pandemic (January 2018-December 2021) who were referred to the National Reference TB Laboratory (NRL TB), Tehran, Iran.
References
More filters
Book
Methods for general and molecular bacteriology
TL;DR: Methodology for General and Molecular Microbiology Morphology Light microscopy Determinative and cytological light microscopy Electron microscopy Cell fractionation Antigen-antibody reactions Growth: Physicochemical factors in growth Nutrition and media Enrichment and isolation Solid, liquid/solid and semisolid culture Liquid culture Growth measurement Culture preservation Molecular Genetics: Gene mutation Gene transfer in Gram-negative bacteria Gene transferIn Gram-positive bacteria Plasmids Transposon mutagenesis
Journal ArticleDOI
Factors Influencing Numbers of Mycobacterium avium, Mycobacterium intracellulare, and Other Mycobacteria in Drinking Water Distribution Systems
TL;DR: Mycobacterial numbers were substantially higher in the distribution system samples (average, 25,000-fold) than in those collected immediately downstream from the treatment facilities, indicating that mycobacteria grow in the Distribution system.
Journal ArticleDOI
Surrounded by mycobacteria: nontuberculous mycobacteria in the human environment.
TL;DR: The widespread distribution of the nontuberculous mycobacteria in the environment, coupled with their ability to degrade and metabolize a variety of complex hydrocarbons including pollutants, suggests that NTM may be agents of nutrient cycling.
Journal ArticleDOI
Nontuberculous mycobacteria in the environment
TL;DR: It is likely that the incidence of infection by environmental opportunistic mycobacteria will continue to rise, part of the rise will be caused by the increased awareness of these microbes as human pathogens and improvements in methods of detection and culture.
Journal ArticleDOI
Two-laboratory collaborative study on identification of mycobacteria: molecular versus phenotypic methods.
TL;DR: It is concluded that molecular typing by 16S rRNA sequence determination is not only more rapid (12 to 36 h versus 4 to 8 weeks) but also more accurate than traditional typing.