Process for purification of industrial waste waters from perchlorates and chlorates

TLDR: In this article, a strain of the microorganism Vibrio dechloraticans Cuznesove B-1168 was grown by successive inoculations on a liquid nutrient medium containing sources of carbon, nitrogen, and phosphorus under anaerobic conditions in the presence of a perchlorate as a donor of oxygen.

Abstract: The process of the present invention involves intermixing of industrial waste waters with household-communal waste waters with subsequent biochemical reduction of perchlorates and chlorates contained in said waters under anaerobic conditions by means of a strain of the microorganism Vibrio dechloraticans Cuznesove B-1168 grown by way of successive inoculations on a liquid nutrient medium containing sources of carbon, nitrogen, and phosphorus under anaerobic conditions in the presence of a perchlorate as a donor of oxygen; said strain having the following morphological characteristics and physiological properties: single cells, size 0.8-1×0.5-0.4μ mobile with one flagella of 1.5-2μ, bent-vibrio, sporeless, well-coloured with fuchsione and non-coloured with methylene blue, gram-negative; grows only on a liquid nutrient medium; does not grow on a meat-pentone agar or agarized medium; can be grown on an acetate under anaerobic conditions in the presence of a perchlorate; well-grown on ethanol with reduction of a perchlorate; lactose, starch, and salts of oxalic and citric acids cannot be used for reduction of perchlorates; glucose and sugars may be used but with diffuclties and only in the presence of a small amount of an acetate; reduces perchlorates and chlorates without dissolved gaseous oxygen in the presence of readily-oxidized organic substances; in minor amounts is capable of assimilating carbonic acid in proportion to the biomass growth; in small amounts capable of reducing nitrates to nitrites, i.e. an enzyme such as nitratereductase is present in cells; reduces perchlorates to chlorides at a rate of 5 g of a perchlorate for 3 days per 1 g of the biomass solids and may grow at a concentration of ammonium perchlorate of about 300 mg/l.