Production, purification, characterization and over-expression of xylanases from actinomycetes.

TLDR: This review provides the state-of-art information and developments on the xylanases from actinomycetes, presenting the production, purification, characterization and over-expression from various act inomycETes cultures.

Abstract: Summary and future perspectives From the above, it is evident that actinomycetes xylanases offer potential industrial applications, in particular for paper and pulp industry, and food and feed industry. Evidently Actinomycetes could be unique source of novel xylanases. Since the wild type strains do not show all the desired properties making them ideal for industrial applications (e.g. high p H and temperature optima together with high enzyme activities for paper and pulp industry), it would be necessary to adopt the biotechnological tools for strain improvement. Streptomyces strains are less amenable to transformation but the use of the techniques such as electrotransformation has paved the way for genetic engineering of the organism. Recombinant DNA techniques can be readily applied to actinomycetes, particularly for the construction of recombinant Streptomyces strain, which produce the enzyme capable of complete solubilisation of the lignocellulose. The understanding of actinomycetes molecular genetics can help further to do alterations in such a way that it becomes a suitable candidate for industrial application. Thus, the improvement of the strains by recombinant DNA technology and site-directed mutagenesis appear to be the most promising options and should be explored in future works.