Journal ArticleDOI
Properties of NAD glycohydrolase purified from five-pace snake (Agkistrodon acutus) venom.
TLDR
NAD glycohydrolase (NADase) from five-pace snake venom was purified to electrophoretic homogeneity through a 4-step isolation procedure, including column chromatography, and showed maximal activity at about 40 degrees C with optimal pH at 7.2.About:
This article is published in Toxicon.The article was published on 1988-01-01. It has received 9 citations till now. The article focuses on the topics: Enzyme complex & Sephadex.read more
Citations
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Journal ArticleDOI
Ophidian envenomation strategies and the role of purines
TL;DR: It is concluded that the principal function of L-type Ca(2+) channel antagonists and muscarinic toxins, in Dendroaspis venoms, and acetylcholinesterase in other elapid venom, is to promote hypotension.
Journal ArticleDOI
Metal ions binding to NAD-glycohydrolase from the venom of Agkistrodon acutus: Regulation of multicatalytic activity
TL;DR: Interestingly, each tested metal ion has a very similar activation of both NADase and ADPase activities, suggesting that the two different activities probably occur at the same site.
Journal ArticleDOI
Small-molecule reductants inhibit multicatalytic activity of AA-NADase from Agkistrodon acutus venom by reducing the disulfide-bonds and Cu(II) of enzyme.
TL;DR: G glutathione and L-ascorbate are endogenous inhibitors to the multicatalytic activity of AA-NADase, suggesting both ADPase and NADase activities probably occur at the same site.
Journal ArticleDOI
Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus.
TL;DR: It is shown that AA-NADase is not only able to cleave the C-N glycosyl bond of NAD to produce ADPR and nicotinamide, but also able to Cleave the phosphoanhydride linkages of ATP, ADP and AMP-PNP to yield AMP.
Journal ArticleDOI
Purification of NAD glycohydrolase from Agkistrodon acutus venom
TL;DR: NAD glycohydrolase (NADase) from Agkistrodon acutus venom was purified to electrophoretic homogeneity by a fast, reproducible 3-step procedure including Q Sepharose Fast Flow, Superdex 75, and Mono S column chromatography.
References
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Journal ArticleDOI
Colorimetric Method for Determination of Sugars and Related Substances
TL;DR: In this article, a method was developed to determine submicro amounts of sugars and related substances using a phenol-sulfuric acid reaction, which is useful for the determination of the composition of polysaccharides and their methyl derivatives.
Journal ArticleDOI
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
Journal ArticleDOI
Estimation of the molecular weights of proteins by Sephadex gel-filtration.
TL;DR: The results are similar to those of previous studies, where the objective was to establish a cause-and-effect relationship, rather than a straightforward relationship between the number of cells and the content of the molecule.
Journal ArticleDOI
Spectroscopic determination of tryptophan and tyrosine in proteins.
TL;DR: A procedure is presented which strongly reduces or elimi- nates these interactions, normalizes their absorption, and consequently permits a more precise analysis of tryptophan and tyrosine in proteins.
Journal ArticleDOI
Glycoproteins of Cell Surfaces A COMPARATIVE STUDY OF THREE DIFFERENT CELL SURFACES OF THE RAT
TL;DR: Each membrane is found to have an identifiable glycoprotein subunit pattern composed of at least 6 to 11 different sized subunits, and most of the staining intensity is present in one to three subunits.
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