Properties of the insulin receptor of isolated fat cell membranes.
TLDR
Modification of the membranes with several protein reagents suggests that tyrosyl and possibly histidyl residues may be important in the binding interaction, and no evidence is present for the involvement of sulfhydryl, tryptophanyl, or carboxyl groups of the membrane.About:
This article is published in Journal of Biological Chemistry.The article was published on 1971-12-10 and is currently open access. It has received 211 citations till now. The article focuses on the topics: Insulin receptor & Membrane.read more
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Book ChapterDOI
Insulin: The Structure in the Crystal and its Reflection in Chemistry and Biology by
TL;DR: The physical, chemical, and biological properties of insulin in the light of the atomic arrangement found in insulin crystals are reviewed in this paper, where the relation of the three-dimensional arrangement of the atoms in the molecule of 2-zinc insulin crystal to the solution properties of the insulin (particularly its states of aggregation), to the chemical reaction and chemical modification of the molecule, and to its primary biological activity is discussed.
Book ChapterDOI
Membrane receptors and hormone action.
TL;DR: This chapter describes the relation between biological activity and binding, physicochemical detection and assay of receptors, solubilization of membrane receptors, examples of membrane-localized receptors, affinity techniques, polyvalent hormone derivatives, and receptor studies, and theoretical mechanisms of hormone–receptor binding and action.
Journal ArticleDOI
Binding and degradation of 125I-insulin by rat hepatocytes.
S Terris,Donald F. Steiner +1 more
TL;DR: Observations suggest that insulin bound to hepatocyte plasma membranes is the substrate for insulin degradation by the liver.
Journal ArticleDOI
Insulin-Receptor Interaction in the Obese-Hyperglycemic Mouse: A MODEL OF INSULIN RESISTANCE
TL;DR: The decrease in insulin receptors in the ob/ob mouse correlates well with the insulin resistance which they exhibit and Scatchard analysis suggests that this decrease in binding is due to a decrease in the number of receptor sites in the membrane of the obese mouse, especially those of higher affinity.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Metabolism of isolated fat cells. i. effects of hormones on glucose metabolism and lipolysis.
The Metabolism of Isolated Fat Cells: I. Effects of Hormones on Glucose Metabolism and Lipolysis
TL;DR: This article marks the beginning of Rodbell's interest in cell receptors and related his discovery that fat cells could be isolated from other cells by treating them with preparations of collagenase, and also found that insulin could stimulate glucose uptake.
Journal ArticleDOI
A Method for the Quantitative Modification and Estimation of Carboxylic Acid Groups in Proteins
D.G. Hoare,Daniel E. Koshland +1 more
TL;DR: The kinetics of the reaction indicates that there are limits to the variation of carbodiimide and nucleophile when quantitative modification is desired, but that a wide variety of reagents can be used if quantitative reaction is not essential.