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Purification and Properties of Oxalate Oxidase from Barley Seedlings

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TLDR
In this paper, a barley seedlings (Hordeum vulgare L. var. distichon ALEFELD) was purified to homogeneity as determined by disc gel electrophoresis.
Abstract
Oxalate oxidase from barley seedlings (Hordeum vulgare L. var. distichon ALEFELD) was purified to homogeneity as determined by disc gel electrophoresis. The enzyme hydrolyzed oxalate, but no other related acids. The optimum pH was around pH 3.5, and this enzyme was stable at acidic pHs. The enzyme appears to consist of two identical subunits. Various other properties were investigated.

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Overexpression of a gene encoding hydrogen peroxide-generating oxalate oxidase evokes defense responses in sunflower

TL;DR: Transgenic sunflower plants constitutively expressing a wheat OXO gene exhibited enhanced resistance against the OA-generating fungus Sclerotinia sclerotiorum and GeneCalling, an RNA-profiling technology, was used to analyze the alteration of gene expression in the transgenic plants.
Journal ArticleDOI

Biological detoxification of fungal toxins and its use in plant breeding, feed and food production.

TL;DR: Current application of research on the biological detoxification of fungal toxins by microorganisms and plants and its practical applications in food and feed production and plant breeding is summarized and expected future developments are outlined.
Journal ArticleDOI

Oxalate, germin, and the extracellular matrix of higher plants.

TL;DR: The article assembles and elaborates evidence which indicates that an ‘old’ enzyme, oxalate oxidase, and an even ‘older’ substrate, calcium Oxalate, have significant and previously uncontemplated roles in the biochemistry of the extracellular matrix (ECM) of higher plants.
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Soybean plants expressing an active oligomeric oxalate oxidase from the wheat gf-2.8 (germin) gene are resistant to the oxalate-secreting pathogen Sclerotina sclerotiorum☆

TL;DR: This is the first report of plant resistance to the fungal pathogen S. sclerotiorum in transgenic plants expressing OxO, and transgenic soybean plants produced byAgrobacterium -mediated transformation with the wheat germin gene encoding an oligomeric protein, oxalate oxidase (OxO), which oxidizes oxalic acid to carbon dioxide and hydrogen peroxide.
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