Purification and Some Properties of Carnitine Dehydrogenase from Xanthomonas translucens

TLDR: The purification and some prepertics of the carnitine dehydregenase ot' X. transtucens were described and the properties of carnitines dehydrogenasc were clarified.

Abstract: clarify the properties of carnitine dehydrogenasc, In this report, we described the purification and some prepertics of the carnitine dehydregenase ot' X. transtucens. X. translucens (IFO 13558) was grown in a medium containing 20,Og of DL-carnitine chloride, 1.0g of K,HPO,, 1,Og of KH,Pe,, O.5g of MgSO,・7H,O, and e.5g of yeast extract in 1000ml of distilled water at pH 7.0. The cu}tivation was done at 30\"C for 20hr with reciprocal shaking. The harvested cells wcre suspended in 50mM Tris-HCI bufler (pH 7.S) and disrupted with an ultrasonie oscillator, Thc ccll-free extract was addeci te ammonium sulfate to 40% saturation and the precipitate was discarded. The precipitate formed by th¢ further addition of ammonium sulfate to 6e% saturation was collected and dissolved in 50mM Tris-HCI bufflrr (pH 7.5). The・dialyzed enzyme solution xrp'as put on a DEAE-cellulose column (4,0 × 55cm) equilibrated with 50mM Tris-HCI buffer (pH 7.S). The enzymc was eluted with a linear gradient of KCI CO -・O,5M)