Quantitative investigations of idiotypic antibodies : iii. persistence and variations of idiotypic specificities during the course of immunization
TLDR
Since the donor rabbits were challenged repeatedly with antigen, it appears that, after month 8, a portion of the antigen was utilized to stimulate existing cell lines and a portion to initiate new clones.Abstract:
Changes and persistence of idiotypic specificities of specifically purified rabbit anti- p -azobenzoate antibodies were studied by quantitative methods. In each rabbit idiotypes identified 2 months after the start of immunization were still present in comparable concentrations 2 months later. After month 4, they were replaced by new and unrelated specificities; the changes were abrupt in two rabbits and gradual in the third, and were associated with an increase in the average affinity for specific hapten. In two surviving rabbits the new sets of specificities persisted in part for at least 1 yr. Quantitative changes occurred during this period, and the antibody preparation used as immunogen reacted most effectively with the homologous anti-D serum. The antibody population present at month 17 (D17) in one rabbit was deficient in idiotypic specificities present in D8 and lacked specificities present in D2, indicating the presence in D17 of a third group of specificities. The percentage of the antibody population from each rabbit reactive with homologous anti-idiotypic serum was greater at month 8 than at month 2, suggesting a decrease in heterogeneity.
Since the donor rabbits were challenged repeatedly with antigen, it appears that, after month 8, a portion of the antigen was utilized to stimulate existing cell lines and a portion to initiate new clones.
Precipitation of anti- p -azobenzoate antibodies removed idiotypic specificities, indicating that they were not present on the anti-bovine γ-globulin antibodies in the same sera.read more
Citations
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Book ChapterDOI
Expression and function of idiotypes of lymphocytes.
TL;DR: This chapter summarizes a series of new procedures for the production of anti-idiotypic reagents and discusses the serological analysis of antigen receptors on lymphocytes and the functional aspects of lymphocyte receptor idiotypes within an immune network.
Journal ArticleDOI
Implications of the presence of an internal image of the antigen in anti-idiotypic antibodies: Possible application to vaccine production
Alfred Nisonoff,Edmundo Lamoyi +1 more
TL;DR: The possibility is discussed of obtaining monoclonal anti-idiotypic antibodies with epitopes similar to those on an infectious agent that might elicit protective antibodies when inoculated and thus act as a vaccine.
Journal ArticleDOI
Suppression of idiotypic specificities in adult mice by administration of antiidiotypic antibody
TL;DR: Administration of rabbit antiidiotypic antibody directed to A/J antiphenylarsonate antibody suppresses almost completely the subsequent production of antibody of the corresponding idiotype, consistent with central suppression of production of the idiotypic antibodies mediated through interaction with immunoglobulin receptors on lymphocytes.
Journal ArticleDOI
Idiotype suppression. I. Influence of the dose and of the effector functions of anti‐idiotypic antibody on the production of an idiotype
TL;DR: Parameters that influence the effect of passively administered anti‐idiotypic antibody on the expression of idiotype‐positive and of idiotypes‐negative antibodies were investigated in adult A/J mice immunized with Group A streptococci.
Journal ArticleDOI
Studies of idiotypic antibodies : production and characterization of autoantiidiotypic antisera
TL;DR: Results showed that each individual responded to its own F(ab')2 and the antisera were specific for antihapten antibodies of that individual, and inhibition assays showed that the reaction was specifically inhibitable with hapten.
References
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Alfred Nisonoff,David Pressman +1 more
TL;DR: Specifically purified anti- p - azo benzoate antibody from individual rabbits exhibits a large degree of heterogeneity with respect to free energy of combination with p -iodobenzoate, as measured by equilibrium dialysis.
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