Journal ArticleDOI
Rapid detection and identification of pathogenic fungi by polymerase chain reaction amplification of large subunit ribosomal DNA.
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TLDR
A polymerase chain reaction (PCR) based approach to the detection and identification of pathogenic fungi which has potential for the diagnosis of systemic mycoses and species-specific primers which only amplified homologous DNA are described.Abstract:
We describe a polymerase chain reaction (PCR) based approach to the detection and identification of pathogenic fungi which has potential for the diagnosis of systemic mycoses. Primers to sequences of the large subunit ribosomal DNA genes, which are universally conserved within the fungal kingdom, were capable of amplifying DNA from 43 strains representing 20 species (12 genera) of medically important fungi. Sequence analysis of the products obtained from Aspergillus fumigatus, Candida albicans and Cryptococcus neoformans allowed us to design species-specific primers which only amplified homologous DNA. The use of these two PCRs in tandem allows the detection (universal PCR) and identification (species-specific PCR) of a fungal pathogen within 8 h from simulated clinical specimens.read more
Citations
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Biodiversity and systematics of basidiomycetous yeasts as determined by large-subunit rDNA D1/D2 domain sequence analysis.
TL;DR: The molecular systematics of 337 strains of basidiomycetous yeasts and yeast-like fungi, representing 230 species in 18 anamorphic and 24 teleomorphic genera, was determined by sequence analysis of the D1/D2 region of the large-subunit rDNA.
Journal ArticleDOI
Detection and identification of fungal pathogens in blood by using molecular probes.
Hermann Einsele,Holger Hebart,G. Roller,Jürgen Löffler,I. Rothenhöfer,C. A. Müller,R. A. Bowden,J.-A. Van Burik,D. Engelhard,Lothar Kanz,Ulrike Schumacher +10 more
TL;DR: Preliminary data from the screening of a selected group of patients revealed some value in the early diagnosis and monitoring of antifungal therapy and the described PCR assay allows for the highly sensitive and specific detection and identification of fungal pathogens in vitro and in vivo.
Journal ArticleDOI
Detection and Identification of Fungal Pathogens by PCR and by ITS2 and 5.8S Ribosomal DNA Typing in Ocular Infections
TL;DR: Whether sequence analysis of internal transcribed spacer/5.8S ribosomal DNA can be used to detect fungal pathogens in patients with ocular infections and shows potential as a rapid technique for identifying fungi in ocular samples is determined.
Journal ArticleDOI
Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections
Siew Fah Yeo,Brian Wong +1 more
TL;DR: The current status and recent developments as well as problems in the design of nonculture diagnostic methods for invasive fungal infections are reviewed.
Journal Article
Laboratory diagnosis of invasive candidiasis.
TL;DR: Diagnostic tests currently in use as well as those under development are compared by describing their assets and limitations for the diagnosis of invasive candidiasis.
References
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Journal ArticleDOI
Detection of a wide range of medically important fungi by the polymerase chain reaction.
TL;DR: PCR analysis of blood specimens collected from mice systemically infected with C. albicans and clinical samples including blood, cerebrospinal fluid and sputum appeared to be a more sensitive diagnostic method for invasive fungal infections than a conventional blood culture technique.
Journal ArticleDOI
New spectrum of fungal infections in patients with cancer.
Elias Anaissie,Gerald P. Bodey,Hagop M. Kantarjian,Jae Y. Ro,Shahe Vartivarian,Roy L. Hopfer,Jennifer F Hoy,Kenneth V. I. Rolston +7 more
TL;DR: Fungal sinusitis, previously caused by Aspergillus species and the phycomycetes, also occurs as a result of some of these newly recognized fungi, suggesting that unusual fungi have now emerged as significant pathogens in this patient population.
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The Growing Problem of Mycoses in Patients Infected with the Human Immunodeficiency Virus
TL;DR: With the rapid increase in cases of AIDS over the past 10 years, certain mycoses have dramatically risen in frequency, particularly those contained by T cell-mediated mechanisms of host defense, which has stimulated trials of new antifungal agents and regimens particularly designed to facilitate long-term outpatient management of mycose without interfering with treatment of either HIV infection itself or other concomitant complications.
Journal ArticleDOI
The detection of Aspergillus spp. by the polymerase chain reaction and its evaluation in bronchoalveolar lavage fluid.
TL;DR: PCR-based detection of fragments of genes-encoding alkaline proteases from Aspergillus fumigatus and A. flavus in bronchoalveolar lavage fluid specimens may prove useful in the diagnosis of invasive pulmonary aspergillosis.
Journal ArticleDOI
Detection of Aspergillus fumigatus by polymerase chain reaction.
TL;DR: The results indicate that PCR detection might be a valuable adjunct to current laboratory methods to diagnose IA and that isolation of Aspergillus spp.