Open AccessJournal Article
Rapid Detection of Australia Antigen by Counterimmunoelectrophoresis
David J. Gocke,Calderon Howe +1 more
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TLDR
With this technique small amounts of antigen with relatively rapid electrophoretic mobility can be detected by specific precipitation with antibody in 1 hr, which is at least 10 times as sensitive as the Ouchterlony double diffusion method.Abstract:
This report describes the detection of Australia antigen by counterelectrophoresis in agar gel. With this technique small amounts of antigen with relatively rapid electrophoretic mobility can be detected by specific precipitation with antibody in 1 hr (1, 2). In addition to its speed the technique is at least 10 times as sensitive as the Ouchterlony double diffusion method. Materials and Methods. Sera containing Australia antigen were obtained from patients with acute viral hepatitis. Specific antisera were from hemophiliacs who had received numerous blood transfusions and were thus repeatedly exposed to hepatitis virus. The standard sera containing Australia antigen used in this laboratory, as well as the homologous antisera, have been shown to have the same specificities as those employed by Blumberg (3) and Prince (4). Counterelectrophoresis is carried out on Kodak projection slides (3.25 × 4 in) covered with 10 ml of 0.85% Agarose in Veronal buffer, 0.05 M, pH 8.2.read more
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Journal ArticleDOI
The Raji cell radioimmune assay for detecting immune complexes in human sera.
TL;DR: A sensitivie and simple procedure for the detection and quantitation of soluble complement (C)- fixing immune complexes in sera of patients with various disease states has been developed by utilizing C receptors on Raji cells.
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Association between polyarteritis and australia antigen
DavidJ. Gocke,Konrad C. Hsu,Councilman Morgan,Stephano Bombardieri,Michael D. Lockshin,CharlesL. Christian +5 more
TL;DR: Four of eleven patients with biopsyproven polyarteritis nodosa were also found to have Australia (Au) antigenaemia and the presence of circulating immune complexes in the sera of three of the four Au-antigen-positive patients was demonstrated by serological, ultracentrifugal, and electro-microscopic studies.
Journal ArticleDOI
Glomerulonephritis with deposition of Australia antigen-antibody complexes in glomerular basement membrane.
Burton Combes,James Shorey,Arnaldo Barrera,Peter Stastny,EdwinH. Eigenbrodt,AlanR. Hull,NormanW. Carter +6 more
TL;DR: It is suggested that Au antigen was involved in the formation of immune complexes whose glomerular deposition initiated the pathologic process leading to the development of diffuse membranous glomerulonephritis.
Journal ArticleDOI
Identification of antibodies to nuclear acidic antigens by counterimmunoelectrophoresis
N Kurata,Eng M. Tan +1 more
TL;DR: CIE is a rapid and sensitive technique for detecting precipitating antibodies to a number of nuclear acidic antigens and methods are described to identify the immunochemical specificities of the precipitin lines by the use of standard reference sera.
Journal ArticleDOI
Antibody to hepatitis-b-virus core in man
TL;DR: In 15 patients, antibodies to core appeared in acute viral hepatitis, type B, twelve to twenty weeks after exposure, usually during antigenaemia and well before the appearance of anti-HBAg, and anti-core antibodies were found in all chronic HBAg carriers tested.
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