Journal ArticleDOI
Rapid enzyme-induced hydrolysis of microgram amounts of phosphatidylcholine on phospholipase A2/celite columns
TLDR
A method has been developed to hydrolyze microgram amounts of phosphatidylcholine (PC) regiospecifically and demonstrated consistent selectivity for 14∶0 liberation, with small amounts of 16∷0 probably indicative of acyl scrambling during the synthesis of the PC.Abstract:
A method has been developed to hydrolyze microgram amounts of phosphatidylcholine (PC) regiospecifically. Hydrolysis of thesn-glycerol 2-acyl group occurs rapidly on microcolumns of immobilized phospholipase A2 on Celite 545 diatomaceous earth. Close to 90% reaction occurs within the first 5 min. Acyl group analysis then may be accomplished by gas liquid chromatography (GLC) of the resulting fatty acids. Hydrolysis of α-16∶0-β-14∶0 PC demonstrated consistent selectivity for 14∶0 liberation, with small amounts of 16∶0 probably indicative of acyl scrambling during the synthesis of the PC. Hydrolysis of an equal weight mixture of (14∶0)2 PC and (18∶2)2PC demonstrated nonpartiality of the immobilized enzyme for either a saturated or unsaturated substrate. The new methodology offers a convenient and sensitive alternative to the presently used procedures.read more
Citations
More filters
Progress in the chemistry of fats and other lipids, Vol. 1
TL;DR: This famous book will not become a unity of the way for you to get amazing benefits at all, but, it will serve something that will let you get the best time and moment to spend for reading the book.
Patent
Fuels as solvents for the conduct of enzymatic reactions
TL;DR: In this paper, the authors describe a method of producing bio-fuels by carrying out the enzymatic transesterification of fatty acid-containing materials directly in automotive fuels, which is a method similar to ours.
Journal Article
Nuclear Envelope Alterations Accompanying Thioacetamide-related Enlargement of the Nucleus
TL;DR: Treating rats with thioacetamide results in a biphasic nuclear swelling, with an initial enlargement phase (0 to 8 hr) and a secondary phase (18 to 48 hr) that coincides with and may be responsible for alterations in sedimentation properties of isolated nuclei.
References
More filters
Book
Laboratory techniques in biochemistry and molecular biology
T. S. Work,E. Work +1 more
TL;DR: This book discusses laboratory techniques in biochemistry and molecular biology, as well as some of the applications of these techniques in medicine and food science.
Journal ArticleDOI
A universal reagent for phospholipid analysis.
TL;DR: A simple procedure for preparing a stable stock reagent an working reagents for the detection and determination of phospholipids is proposed, and a simple, rapid and accurate thin-layer chromatographic technique is suggested.
Journal ArticleDOI
Synthesis of lecithins by acylation of O-(sn-glycero-3-phosphoryl) choline with fatty acid anhydrides
E.Cubero Robles,D. Van Den Berg +1 more
TL;DR: A new method has been developed for the partial synthesis of saturated, unsaturated and mixed-acid lecithins using the free O-(sn-glycero-3-phosphoryl) choline and its CdCl2 complex as starting materials.
Journal ArticleDOI
Glycerophospholipid synthesis: improved general method and new analogs containing photoactivable groups.
TL;DR: New phospholipids containing photoactivable groups, such as trifluorodiazopropionyl, diazirinophenoxy, 2-nitro-4-azidophenoxy; and alpha, beta-unsaturated keto groups, in the fatty acyl chains have been prepared.
Related Papers (5)
Nonionic micellar liquid chromatography coupled to immobilized enzyme reactors
Purification of estradiol-17β dehydrogenase from human placenta by affinity chromatography
Chin Chang-chen,James C. Warren +1 more