Ratiometric electrochemical detection of alkaline phosphatase

TLDR: A novel ferrocene-derived substrate for the ratiometric electrochemical detection of alkaline phosphatase (ALP) was designed and synthesised and demonstrated to be an excellent electrochemical substrates for the ALP-labelled enzyme-linked immunosorbent assay (ELISA).

About: This article is published in Chemical Communications.The article was published on 2015-01-11 and is currently open access. It has received 137 citations till now. The article focuses on the topics: Alkaline phosphatase.

Figures

Fig. 1 Differential pulse voltammogram obtained for substrate 1 (100 µM) and ferrocenylamine 3 (100 µM) in 0.05 M pH 9 Tris buffer.

Fig. 3 Conversion of substrate to product after addition of ALP (100 UL - 1 ) using different concentrations of substrate at room temperature.

Fig. 2 Conversion of substrate (100 µM) to product after addition of different concentrations of ALP at room temperature.

Fig. 7 Current measured from CRP ELISA using substrate 1 and AA2P as electrochemical. Error bars indicate standard deviations (n = 6).

Fig. 4 Conversion of substrate (50 µM) to product after addition of different concentrations of ALP at 37 °C. 10

Fig. 5 Amperometric response of ferrocenylamine released from substrate 1 (50 µM) after the addition of ALP in different concentrations. Error bars indicate standard deviations (n = 3). LOD calculated as 3 × standard