Receptors for 1,25(OH)2-vitamin D3 enriched in cloned osteoblast-like rat osteogenic sarcoma cells
Nicola C. Partridge,R. J. Frampton,John A. Eisman,V. P. Michelangeli,E. Elms,T. R. Bradley,T. J. Martin +6 more
Reads0
Chats0
TLDR
In this article, the authors report the development of clones of this osteogenic sarcoma, which have an active adenylate cyclase response to PTH and high alkaline phosphatase activity, and are enriched in a cytosol receptor for the bone resorbing steroid hormone, 1,25 <OH)D3.About:
This article is published in FEBS Letters.The article was published on 1980-06-16 and is currently open access. It has received 164 citations till now. The article focuses on the topics: Bone cell & Osteoblast.read more
Citations
More filters
Journal ArticleDOI
Role of osteoblasts in hormonal control of bone resorption - a hypothesis
Gideon A. Rodan,T. J. Martin +1 more
TL;DR: The following hypothesis attempts to explain the puzzling fact that osteoblasts--the bone-forming cells--seem to be the target cells of parathyroid hormone (PTH), the prostaglandins, and 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), the bone-resorbing hormones.
Journal ArticleDOI
The vitamin D endocrine system: steroid metabolism, hormone receptors, and biological response (calcium binding proteins).
TL;DR: Vitamin D was first identified through the nutritional studies of Sir Edward Mellanby (243), which identified rickets as a disease state resulting from a deficiency of vitamin D, as well as through the biochemical/chemical studies of McCollum and co-workers (242), who demonstrated that there was more than one fat-soluble vitamin.
Journal Article
Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties.
Sevgi B. Rodan,Yasuo Imai,Mark A. Thiede,Gregg Wesolowski,David C. Thompson,Zvi Bar-Shavit,Susan Shull,Kenneth G. Mann,Gideon A. Rodan +8 more
TL;DR: The Saos-2 cells possess several osteoblastic features and could be useful as a permanent line of human osteoblast-like cells and as a source of bone-related molecules.
Journal ArticleDOI
Rapidly forming apatitic mineral in an osteoblastic cell line (umr 106-01 bsp)
TL;DR: Results indicate that UMR 106-01 BSP cells form a bio-apatitic mineralized matrix upon addition of supplemental phosphate, which involves alkaline phosphatase activity, on-going RNA and protein synthesis, as well as Golgi-mediated processing and secretion.
Journal ArticleDOI
Regulation of bone formation.
TL;DR: The osteopenia associated with glucocorticoids is termed osteoporosis because there is no obvious defect in mineralization, so that wide osteoid seams do not .
References
More filters
Journal ArticleDOI
Biochemical Characterization with Parathormone and Calcitonin of Isolated Bone Cells: Provisional Identification of Osteoclasts and Osteoblasts
TL;DR: Two metabolically distinct types of bone cell populations were isolated from mouse calvaria by a repetitive digestive procedure with a mixture of collagenase and trypsin, and it is postulate that the CT type of populations is enriched in osteoclasts and, possibly, osteocytes, and the PT type of population is enrichment in osteoblasts.
Journal ArticleDOI
Oestrogen-responsive human breast cancer in long term tissue culture.
Marc E. Lippman,Gail Bolan +1 more
TL;DR: The characterisation of several human breast cancer cell lines are reported, at least one of which shows marked dependence on oestrogens for growth, which seems to be similar in a variety of ways to that observed clinically in vivo.
Journal ArticleDOI
A specific high-affinity binding macromolecule for 1,25-dihydroxyvitamin D3 in fetal bone
TL;DR: Cytosol fractions were prepared from fetal rat or embryonic chick calvaria and analyzed for binding of vitamin D3 metabolites on sucrose density gradients and 1,25-dihydroxy-[3H]vitamin D3 appears to have greater affinity for the 3.5S component.
Journal ArticleDOI
The effect of oxygen tension on haemopoietic and fibroblast cell proliferation in vitro.
TL;DR: The effects of providing low oxygen tension in the gas phase of two different types of cell culture systems were investigated and the increased efficiency of colony formation was observed both with mouse and human marrow cells.
Journal ArticleDOI
Activation of skeletal adenyl cyclase by parathyroid hormone in vitro.
TL;DR: Adenyl cyclase in calvaria was not sensitive to a variety of hormones known to stimulate this enzyme in other tissues, and these observations provide the basis for the concept that a single type of interaction between hormone and enzyme bound to...