Journal ArticleDOI
Semimicro method for determination of serum uric acid using EDTA-hydrazine.
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TLDR
A linear relationship is obtained upon comparing this method with the sodium carbonate and the differential ultraviolet spectrophotometric methods.Abstract:
A semimicro method is described for the determination of uric acid by manual and automated procedures. Serum uric acid reduces phosphotungstic acid in an alkaline medium of EDTA sodium tungstate and hydrazine sulfate, the latter being used as a color intensifier. The use of sodium tungstate eliminates the development of interfering turbidity encountered with other alkalizing reagents. This method requires only 0.2 ml. of serum. The presence of ascorbic acid, sodium salicylate, cysteine, and/or glucose does not interfere. A linear relationship is obtained upon comparing this method with the sodium carbonate and the differential ultraviolet spectrophotometric methods.read more
Citations
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Journal ArticleDOI
Serum uric acid levels and cancer mortality risk among males in a large general population-based cohort study
TL;DR: This study indicates that elevated SUA levels may protect against cancer mortality, and uses multivariate Cox regression to analyze the association between cancer mortality risk and SUA level.
Journal ArticleDOI
Technical bulletin No. 31. Determination of uric acid in blood and in urine.
TL;DR: The concentration of uric acid may be determined by direct spectrophotometry (following the change in absorbence at 292 nm during the reaction) or by measuring the amount of oxygen consumed or the amounts of hydrogen peroxide produced.
Journal ArticleDOI
Determination of Serum Urate by Direct Acid Fe3+ Reduction or by Absorbance Change (at 293 nm) on Oxidation of Urate with Alkaline Ferricyanide
TL;DR: An acid ferric reduction procedure is described for determining serum urate with 2,4,6-tripyridyl- s -triazine at 593 nm, whereby each mole of urate reduces 4 moles of ferric ion.
Journal ArticleDOI
Miniaturized graphite sensors doped with metal-bathophenanthroline complexes for the selective potentiometric determination of uric acid in biological fluids.
TL;DR: Miniaturized poly(vinyl chloride) matrix membrane sensors in an all-solid-state graphite support, responsive to urate anion, gave results that compared favourably with those obtained by the standard spectrophotometric method.
References
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Journal ArticleDOI
The enzymatic spectrophotometric method for determination of uric acid.
TL;DR: A detailed description of the procedure used in this laboratory for the enzymatic spectrophotometric determination of uric acid in biologic fluids and studies of its accuracy and reproducibility as well as further evidence for its high degree of specificity are presented.
Journal ArticleDOI
A modified carbonate-phosphotungstate method for the determination of uric acid and comparison with the spectrophotometric uricase method.
Journal ArticleDOI
Colorimetric measurement of uric acid.
TL;DR: A convenient modification of the uric acid method devised by Kern and Stransky has been described and its reliability has been demonstrated largely by other workers.
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