Journal ArticleDOI
Sister chromatid exchanges in leukocytes of patients with cancer of cervix uteri
TLDR
A preliminary study indicates the possibility of using SCE as a preclinical marker in cervical cancer cases and the values of cancer cases deviate significantly from that of controls.Abstract:
The frequency of sister chromatid exchange (SCE) was investigated in 13 women with cervical cancer together with 11 control women. The SCE frequencies were found to be 10.05±2.35 and 6.95±1.53 in cancer cases and controls, respectively. The SCE values of cancer cases deviate significantly from that of controls. The SCE in chromosome groups E, F, and G was found to be more in comparison to controls (P<0.001). This preliminary study indicates the possibility of using SCE as a preclinical marker.read more
Citations
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Constitutional chromosome instability and cancer risk.
TL;DR: Although most results thus fail to support constitutional chromosome fragility as a factor of importance in tumorigenesis, conclusive falsification of the hypothesis cannot be said to have been obtained, the possibility remains that variations in chromosome stability and clastogen sensitivity between different cell types, and difficulties in selecting the most appropriate carcinogens in clasts, may have masked systematic constitutional differences between patients and controls in the breakage assays.
Journal ArticleDOI
Chromosome aberrations and sister chromatid exchange studies in patients with prostate cancer : Possible evidence of chromosome instability
TL;DR: The results indicate that the patients with prostate cancer show a degree of chromosomal instability that might be related to a predisposition to neoplasia.
Journal ArticleDOI
Factors that Influence Formation of Sister Chromatid Exchanges in Human Blood Lymphocytes
TL;DR: Physical or preparatory as well as biological factors that modify the response and formation of SCEs make the monitoring difficult and the purpose of this article is to review and analyze these factors to facilitate an effective development of a standard protocol for SCE testing and for appropriate evaluation of test results.
Journal ArticleDOI
Spontaneous chromosomal instability in breast cancer families.
Shambhu K Roy,Amit H. Trivedi,Sonal R Bakshi,Rashmi K. Patel,Pina H Shukla,Shailesh J Patel,Jyotsna M. Bhatavdekar,Devendra D. Patel,Pankaj M. Shah +8 more
TL;DR: The findings primarily indicate the high level of chromosomal instability in breast cancer families, and might be one of the predisposing factors for high risk of cancer in HBR.
Journal ArticleDOI
Sister chromatid exchanges in patients with precancerous and cancerous lesions of cervix uteri
TL;DR: The increased frequencies of SCEs in the majority of cancer patients and a few, precancerous lesions indicate that individuals with high SCE levels may be at a high risk of developing cancer.
References
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Journal ArticleDOI
New Giemsa method for the differential staining of sister chromatids
Paul Perry,Sheldon Wolff +1 more
TL;DR: If human lymphocytes1 or Chinese hamster2 cells are treated with the base analogue 5-bromodeoxyuridine in the latter part of the S period, Giemsa stained chromosomes exhibit a pattern of condensed and extended segments along their length, allowing the identification of the two chromatids, and the observation of sister chromatid exchanges (SCEs) without recourse to autoradiography.
Journal ArticleDOI
Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange.
Paul Perry,H. J. Evans +1 more
TL;DR: A staining technique that detects sister chromatid exchanges (SCEs) has been used to examine the response of chromosomes in cultured Chinese hamster cells to a wide variety of mutagens–carcinogens.
Journal ArticleDOI
Factors involved in differential giemsa-staining of sister chromatids
TL;DR: Microspectrophotometric evaluation of differentially stained sister chromatids made it possible to analyse precisely the factors involved in the Giemsa methods where the photosensitive Hoechst 33258 played a role as a sensitizer.
Journal ArticleDOI
Sister chromatid exchange as an assay for genetic damage induced by mutagen-carcinogens. II. In vitro test for compounds requiring metabolic activation.
Daniel G. Stetka,Sheldon Wolff +1 more
TL;DR: Sister chromatid exchanges (SCE's) which are easily seen by "harlequin chromosome" techniques can be readily induced in cultured Chinese hamster ovary cells by low concentrations of mutagen-carcinogens that do not require metabolic activation.
Journal ArticleDOI
Induction of sister chromatid exchanges by chemical mutagens and its possible relevance to DNA repair.
TL;DR: It was found that 4NQO and MMC exerted remarkable delayed effects on the exchange induction, whereas proflavin did not, which seems to suggest that the lesions caused by the former mutagens would be long-lived and repeatedly provoke sister chromatid exchanges.
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Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange.
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