Journal ArticleDOI
Site of Action of Trophic Hormones Upon the Biosynthetic Pathways to Steroid Hormones
Peter F. Hall,Derek G. Young +1 more
TLDR
Under none of the conditions tested in any of the 3 tissues was the conversion of 20α-hydroxycholesterol-7α-3H to the steroid hormones increased in the presence of the trophic hormones.Abstract:
Slices of bovine adrenal cortex, bovine corpora lutea and rabbit testes were incubated with 20α-hydroxycholesterol-7α-3H or with cholesterol-7α-3H with and without the trophic hormones ACTH (adrenal cortex) or ICSH (corpora lutea or testes) added in vitro. In confirmation of previous findings, the trophic hormones increased the conversion of cholesterol-3H to cortisol-3H (adrenal cortex), progesterone-3H (corpora lutea) and testosterone-3H (testes). On the other hand, the trophic hormones produced a slight decrease in the conversion of 20α-hydroxycholesterol-3H to the same steroid hormones. In the case of the corpus luteum this decrease was less when incubation was continued for longer times, but under none of the conditions tested in any of the 3 tissues was the conversion of 20α-hydroxycholesterol-7α-3H to the steroid hormones increased in the presence of the trophic hormones. In the case of the testis similar results were observed 1 hr after a single intravenous injection of ICSH. These findings were m...read more
Citations
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Journal ArticleDOI
Testicular microsomal cytochrome P-450 for C21 steroid side chain cleavage. Spectral and binding studies.
S Nakajin,Peter F. Hall,M Onoda +2 more
TL;DR: It is concluded that the powerful forward competitive inhibition by pregnenolone and progesterone may be important in regulating synthesis of androgens in vivo and the evidence presented suggests one active site for both hydroxylase and lyase activities.
Book ChapterDOI
Cellular Organization for Steroidogenesis
TL;DR: In reviewing the regulation of steroidogenesis, the chapter points out two major factors—the properties of individual enzymes and their arrangement in lipid membranes; these are important in the microsomal process.
Journal ArticleDOI
The role of lipoproteins in the regulation of progesterone secretion by the human corpus luteum.
Journal ArticleDOI
Response of adrenal tumor cells to adrenocorticotropin: site of inhibition by cytochalasin B.
James J. Mrotek,Peter F. Hall +1 more
TL;DR: Findings indicate that cytochalasin acts after cholesterol has entered the cells and before it is subjected to side-chain cleavage in mitochondria, and that at least one effect of ACTH (and cyclic AMP) is exerted upon this transport process.
Journal ArticleDOI
Mechanism of ACTH action
TL;DR: ACTH and cAMP appear to direct the adrenal cell toward the differentiated, maximally functional state.