Journal ArticleDOI
Steroid biosynthesis in vitro by transplantable interstitial cell tumor of mice. I. Identification and quantitative determination of the metabolites and intracellular distributon of the enzymes related to testosterone formation.
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TLDR
Androgen producing activity of a transplanted tumor of mice, originating in the testicular interstitial cell, was biologically confirmed by the fact that the sex-accessory organs of the tumor-bearing mice did not show any decrease in weight even after removal of thetesticular glands.Abstract:
Androgen producing activity of a transplanted tumor of mice, originating in the testicular interstitial cell, was biologically confirmed by the fact that the sex-accessory organs of the tumor-bearing mice did not show any decrease in weight even after removal of the testicular glands. After slices of the testicular interstitial cell tumor which had been transplanted to the mice were incubated in vitro with relabeled pregnenolone and progesterone, respectively, as substrate, not only 17α-hydroxyprogesterone, androstenedione and testosterone, but also the corticosteroids such as deoxycorticosterone, 18-hydroxy-ll-deoxycorticosterone, corticosterone and 11-deoxycortisol were isolated as the metabolites and identified, suggesting that this tumor retained enzyme functions of producing adrenocorticoids as well as androgen. Furthermore, progesterone which was administered as a substrate was catabolized into 20α-and 20β-hydroxypregn-4-en-3-one and 6β-hydroxyprogesterone. The enzyme activities related to androgen ...read more
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Journal ArticleDOI
Steroidogenesis and cell structure: Biochemical pursuit of sites of steroid biosynthesis
TL;DR: From the information on the sites of steroid transformations in a cell, intracellular transport of steroidal precursors, intermediates, and final products are integrated together with the biosynthetic pathways of the steroid hormone.
Journal ArticleDOI
Lifetime of microsomal cytochrome P-450 and steroidogenic enzymes in rat testis as influenced by human chorionic gonadotrophin
TL;DR: While it is clear that the two cytochrome P-450 dependent hydroxylases involved in steroidogenesis and the 5 α-reductase are under the control of gonadotrophin, it is not clear how 17β-hydroxysteroid dehydrogenase levels are maintained or in what manner the 5α- reductase level is controlled in mature animals.
Journal ArticleDOI
Submicrosomal distribution of adrenal enzymes and cytochrome P-450 related to corticoidogenesis
TL;DR: Substrate specificities of the adrenal submicrosomal enzymes, dynamic analysis of the corticoidogenesis throughout the incubation time, and the relationship between the amounts of enzyme preparations and the products were examined were examined.
Journal Article
Metabolism of Benzo(a)pyrene by the Isolated Perfused Rat Testis
I. P. Lee,J. Nagayama +1 more
TL;DR: Mixedfunction oxidase(s) in rat testis preferentially epoxidate the 9,10 and 7,8 positions of BP, the TCDD-induced shift in metabolic products could be significant in BP-induced mutagenic and/or carcinogenic effects.
Journal Article
Hormonal Regulation of Cytochrome P-450-dependent Monooxygenase Activity and Epoxide-metabolizing Enzyme Activities in Testis of Hypophysectomized Rats
TL;DR: The result suggests that LH-stimulated induction of testicular AHH and EH activities and P-450 contents may be primarily associated with the interstitial cell compartment, and Leydig cells are target cells of LH.
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