Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase
TLDR
The polynucleotide ligase isolated from T4-infected Escherichia coli is shown to bring about repair of breaks in the single strands of bihelical DNA and can also catalyze the joining of DNA duplexes at their base-paired ends.Abstract:
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction occurs when the deoxynucleoside at a 5′-end carries a phosphate group and the complementary deoxynucleoside opposite to it carries a 3′-hydroxyl group. The consequence is the lengthening of the original duplex to form dimers or oligomers depending upon whether one or both ends are base-paired.read more
Citations
More filters
Patent
Methods and Microfluidic Devices for the Manipulation of Droplets in High Fidelity Polynucleotide Assembly
TL;DR: In this article, methods and devices for manipulating droplets on a support using surface tension properties, moving the droplets along a predetermined path and merging two droplets together enabling a number of chemical reactions are provided.
Journal ArticleDOI
Nucleotide sequence of the attenuator region of the histidine operon of Escherichia coli K-12
TL;DR: The attenuator region of the histidine operon of Escherichia coli K-12 has a potential coding capacity for two peptides, one of 16 amino acids and another of 30 amino acids, and a G+C-rich region precedes and follows a possible transcription termination sequence, which are compatible with a model in which active translation of a leader mRNA interferes with transcription termination, thus causing derepression of the Histidine Operon.
Journal ArticleDOI
Exponential Megapriming PCR (EMP) Cloning-Seamless DNA Insertion into Any Target Plasmid without Sequence Constraints
TL;DR: It is shown that EMP cloning has a higher efficiency than restriction-free (RF) cloning, especially for long inserts above 2.5 kb, and enables simultaneous cloning of multiple inserts.
Patent
Microarray Synthesis and Assembly of Gene-Length Polynucleotides
TL;DR: In this article, a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotide synthesized in situ on a microarray platform is described.
Journal ArticleDOI
Deoxyribonucleic acid ligase. A steady state kinetic analysis of the reaction catalyzed by the enzyme from Escherichia coli.
Modorich P,I. R. Lehman +1 more
TL;DR: The rate of release of adenosine 5'-monophosphate from synthetic DNA-adenylate in the absence of NH4+ is greater than the rate of DNA-joining, as expected for a kinetically significant intermediate.